Anti-TCF4/TCF7L2 Monoclonal Antibody for WB, IHC-P, ELISA - Q9NQB0

Core Product Specifications and Parameters

Biological Background: TCF4/TCF7L2 Function and Localization

• Transcription factor 7-like 2, also known as T-cell factor 4 (TCF-4), is a member of the TCF/LEF family of DNA-binding transcription factors.
• Functions as a key effector in the canonical Wnt/β-catenin signaling pathway, regulating gene expression by binding to Tcf motifs (5'-CCTTTGATC-3') in target gene promoters.
• Acts as a transcriptional repressor in the absence of β-catenin (CTNNB1) and as an activator upon β-catenin binding, switching target gene expression.
• Modulates MYC expression by binding to its promoter, and regulates cell-cycle progression; dominant-negative mutants cause G1 arrest.
• Essential for maintaining the epithelial stem-cell compartment of the small intestine, highlighting its role in tissue homeostasis.
• Predominantly localized in the nucleus and PML bodies, consistent with its role in transcriptional regulation.
• Detected in the epithelium of the small intestine (highest at crypt tops), colon, and mammary gland, with elevated expression in colon and mammary carcinomas.
• Subject to phosphorylation and ubiquitin-like conjugation (ISGylation), influencing activity and stability (Keywords: Phosphoprotein, Ubl conjugation, Isopeptide bond).

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (a.a. 1-100), which may allow detection of multiple TCF7L2 isoforms if this region is conserved.
• For Western blot (WB), a band around 68 kDa is expected; validate in human cell lines known to express TCF7L2, such as colorectal cancer cells.
• For immunohistochemistry (IHC-P), antigen retrieval and antibody dilution should be optimized for formalin-fixed paraffin-embedded (FFPE) human tissue sections, particularly intestinal or colon samples where expression is reported.
• For ELISA, consider using recombinant TCF7L2 protein or peptide fragments covering the immunogen sequence for standard curve generation.
• Since TCF7L2 activity is context-dependent (Wnt pathway), co-treatment with Wnt agonists or β-catenin stabilizers may be considered to study activation states.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with streamlined access to high-quality IVD raw materials, including validated monoclonal antibodies like this anti-TCF4/TCF7L2 clone for Wnt pathway and oncology applications. Our portfolio extends to antibody pairs, optimized antigens, and ancillary reagents, with dedicated support for rare target sourcing and custom development projects, ensuring a reliable supply chain from concept to clinic.