Anti-TCF4 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P15884

Core Product Specifications and Parameters

Biological Background: Transcription Factor 4 (TCF4) Function and Localization

• TCF4, also known as ITF-2 or SEF-2, is a class B basic helix-loop-helix (bHLH) transcription factor encoded by the TCF4 gene (Gene ID: 6925).
• It binds to the immunoglobulin enhancer Mu-E5/KE5-motif and E-box sequences (5'-CANNTG-3'), preferentially 5'-ACANNTGT-3' or 5'-CCANNTGG-3', to activate transcription.
• TCF4 is involved in the initiation of neuronal differentiation and is critical for neurogenesis.
• It localizes to the nucleus and is expressed in adult heart, brain, placenta, skeletal muscle, and lung, with prominent expression in developing embryonic brain.
• The protein undergoes alternative splicing, generating multiple isoforms with distinct functions.
• TCF4 is implicated in several diseases, including Pitt-Hopkins syndrome (PTHS), Fuchs endothelial corneal dystrophy, and epilepsy, as indicated by its association with disease variants.
• Post-translational modifications include phosphorylation, and it is identified in proteomics studies.

Experimental Guidance and Technical Tips

• For Western blot, the expected band is around 71 kDa; consider using nuclear extracts or whole cell lysates from brain or heart tissues where TCF4 is enriched.
• In IF/ICC, validate nuclear localization and use appropriate fixation/permeabilization protocols.
• The antibody detects multiple isoforms due to alternative splicing; additional bands may appear.
• For ELISA, consider using the recombinant immunogen as a positive control to assess binding.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides IVD manufacturers and research institutes with one-stop access to validated antibody pairs, optimized polyclonal antibodies, and bulk ancillary reagents. For transcription factor studies like TCF4, we offer comprehensive sourcing solutions to accelerate diagnostic development from concept to clinic. Explore our catalog for rare-target raw materials and technical support.