Anti-TCF4 Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P15884

Core Product Specifications and Parameters

Biological Background: TCF4 Function and Localization

• Transcription factor 4 (TCF4), also known as immunoglobulin transcription factor 2 (ITF-2) and SEF-2, is a member of the class B basic helix-loop-helix (bHLH) protein family (bHLHb19). It plays a critical role in transcriptional regulation and neuronal differentiation.
• Binds to the immunoglobulin enhancer Mu-E5/KE5-motif and activates transcription by recognizing E-box sequences (5'-CANNTG-3'), with a preference for either 5'-ACANNTGT-3' or 5'-CCANNTGG-3'. It also binds to the E-box in the somatostatin receptor 2 initiator element (SSTR2-INR) (By similarity).
• Involved in the initiation of neuronal differentiation, consistent with its expression in developing embryonic brain and adult brain, heart, placenta, and skeletal muscle.
• Localizes to the nucleus, where it exerts its DNA-binding and transcription regulation functions.
• Post-translational modifications include phosphorylation, and the protein undergoes alternative splicing, generating multiple isoforms.
• Associated with various diseases, including corneal dystrophy, epilepsy, intellectual disability, and primary microcephaly, as indicated by disease variant annotations.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 1–210 of human TCF4 (N-terminal region). For Western blot, the predicted molecular weight is ~71 kDa; however, alternative splicing or post-translational modifications may result in additional bands.
• IHC-P and IF/ICC: Nuclear staining is expected. Consider adequate fixation and permeabilization protocols for optimal antigen detection.
• Due to the polyclonal nature and bHLH domain homology, potential cross-reactivity with other bHLH proteins should be evaluated. Use of knockout or knockdown controls is recommended.
• ELISA: Suitable for capture or detection of TCF4 protein. Validate the assay conditions in the relevant sample matrix.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a trusted one-stop sourcing partner for IVD raw materials, supporting diagnostic manufacturers, laboratories, and research institutes from concept to clinic. For studies focused on TCF4-mediated transcriptional regulation, neuronal differentiation, and related disease pathways, we offer validated polyclonal antibodies, optimized recombinant proteins, and ancillary reagents for bulk procurement. Our team also specializes in rare target raw-material sourcing, ensuring a reliable supply chain for your diagnostic development and translational research needs.