Anti-TCF3 Rabbit Polyclonal Antibody for WB, ELISA - P15923

Core Product Specifications and Parameters

Biological Background: TCF3 Function and Localization

• Encodes transcription factor E2-alpha, a class B basic helix-loop-helix (bHLH) protein also known as TCF3, bHLHb21, or immunoglobulin enhancer-binding factor E12/E47.
• Functions as a transcriptional regulator that initiates neuronal differentiation and promotes mesenchymal to epithelial transition by forming heterodimers with tissue-specific bHLH proteins.
• Binds to E-box DNA motifs (5'-CANNTG-3') and regulates tissue-specific gene expression during embryogenesis, including in muscle and early B-cell differentiation.
• Recognizes the kappa-E2 site in the immunoglobulin gene enhancer, implicating it in B-cell development. Related references: PMID:2493990
• Interacts with the insulin gene transcription control region, contributing to pancreatic cell regulation.
• Enhances ATOH7 binding to the consensus sequence 5'-CAGGTG-3', positively modulating transcriptional activity.
• Localizes predominantly to the nucleus, consistent with its role as a DNA-binding transcription factor.
• Post-translationally modified by phosphorylation and methylation; classified as a proto-oncogene due to its involvement in chromosomal rearrangements associated with leukemia.

Experimental Guidance and Technical Tips

• For Western blot, consider using whole cell lysates and validating the observed band around 68 kDa against the predicted molecular weight.
• The immunogen corresponds to amino acids 450–525 of human TCF3; verify specificity in your target tissue or cell line, as alternative splicing may produce isoforms.
• Due to high sequence conservation across human, mouse, and rat, this antibody may be suitable for cross-species studies; however, validate performance in each species of interest.
• In ELISA applications, titrate the antibody and include appropriate controls to ensure linearity and specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports researchers and diagnostic developers with one-stop access to IVD raw materials, from concept to clinic. This TCF3 polyclonal antibody exemplifies our supply of validated antibodies for transcription factor and developmental biology research. Beyond individual antibodies, we offer matched antibody pairs, bulk ancillary reagents, and rare target raw-material sourcing to streamline your assay development and manufacturing workflows.