Anti-TCF21 (POD1/bHLHa23) Rabbit Polyclonal Antibody for WB and ELISA - O43680

Core Product Specifications and Parameters

Biological Background: TCF21 Function and Localization

• TCF21 (Transcription factor 21), also known as Capsulin, Epicardin, Pod-1, and bHLHa23, is a basic helix-loop-helix (bHLH) transcription factor encoded by the TCF21 gene (POD1/BHLHA23).
• It plays a key role in epithelial-mesenchymal interactions during kidney and lung morphogenesis, driving epithelial differentiation and branching morphogenesis.
• TCF21 may contribute to the specification or differentiation of subsets of epicardial cell types, suggesting a role in cardiac development.
• The protein localizes to the nucleus, where it exerts its transcriptional regulatory functions.
• According to UniProt, TCF21 is annotated with keywords including DNA-binding, transcription regulation, and nucleus, and is part of the reference proteome.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 30-100 of human TCF21, covering an N-terminal region outside the bHLH domain; this design may be useful for detecting native and denatured forms of the protein in WB and ELISA applications.
• For WB, consider using nuclear-enriched lysates from kidney or lung cell lines (e.g., HEK293, A549) or tissues where TCF21 is expressed, and validate the expected band at approximately 20 kDa.
• In ELISA, the antibody can be employed for direct or indirect antigen detection; however, optimal coating conditions and detection ranges should be empirically determined.
• Given the reported cross-reactivity with mouse, this antibody may be suitable for translational studies using murine models of kidney and lung development, but cross-species reactivity should be confirmed in your specific experimental context.

CamelBio: Your One-Stop Sourcing Bridge

As a specialized IVD raw-material partner, CamelBio connects diagnostic manufacturers and research teams with carefully sourced antibodies like this anti-TCF21 polyclonal antibody, along with validated antibody pairs, optimized monoclonals, bulk ancillary reagents, and rare target sourcing. Our focus on transcription factors and developmental biology markers supports the development of robust immunoassays for kidney, lung, and cardiovascular disease research. Reach out to explore how our one-stop supply chain can accelerate your diagnostic development pipeline.