Anti-TCF12 Rabbit Polyclonal Antibody for WB, ELISA - Q99081

Core Product Specifications and Parameters

Biological Background: TCF12 Function and Localization

• TCF12 (Transcription factor 12) is a member of the basic helix-loop-helix (bHLH) family of transcription factors, also known as HEB, HTF4, and bHLHb20.
• It functions as a transcriptional regulator that activates transcription by binding to the E-box motif (5'-CANNTG-3') on target gene promoters (By similarity).
• Involved in the initiation of neuronal differentiation, playing a critical role in the development of the gonadotropin-releasing hormone (GnRH) axis. Related references: PMID:32620954
• Localizes predominantly to the nucleus, consistent with its role in transcriptional regulation.
• TCF12 is associated with diseases such as craniosynostosis, hypogonadotropic hypogonadism, and Kallmann syndrome, and is marked by keywords including alternative splicing, phosphoprotein, and Ubl conjugation.
• Expression is detected in several tissues including skeletal muscle, thymus, and B-cell lines.

Experimental Guidance and Technical Tips

• For Western blot, consider using lysates from human, mouse, or rat tissues such as skeletal muscle or thymus, where TCF12 is expressed. The predicted molecular weight is 73 kDa; verify appropriate band migration.
• In ELISA, consider using the recombinant immunogen as a positive control to assess antibody binding.
• This polyclonal antibody recognizes multiple epitopes; for optimal results, perform titration experiments to determine the best working dilution for your specific assay conditions.
• Note that TCF12 is a nuclear protein; appropriate nuclear extraction or permeabilization may enhance detection in cell-based assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with streamlined access to high-quality IVD raw materials, including this polyclonal antibody targeting TCF12 for studies in neurogenesis and related disorders. Our portfolio includes validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents, supported by rare-target sourcing capabilities to accelerate your diagnostic development.