Anti-TCERG1 Polyclonal Antibody for WB, ELISA - O14776

Core Product Specifications and Parameters

Biological Background: TCERG1 Function and Localization

• TCERG1 (Transcription elongation regulator 1), also known as CA150 or TAF2S, is a transcription factor that directly binds RNA polymerase II and regulates transcription elongation.
• It functions as a repressor by inhibiting elongation of transcripts from target promoters in a TATA box-dependent manner.
• TCERG1 is essential for TAT-dependent activation of the HIV-1 promoter, playing a critical role in viral gene expression.
• The protein localizes exclusively to the nucleus, consistent with its transcriptional regulatory functions.
• Detected in brain neurons, indicating tissue-specific roles in neural gene expression.
• Post-translational modifications, including phosphorylation, methylation, and ubiquitin-like conjugation, regulate its activity; alternative splicing generates multiple isoforms.
• Contains structural motifs such as coiled-coil domains and repeats, facilitating protein-protein interactions.

Experimental Guidance and Technical Tips

• The immunogen corresponds to an internal region (aa 550-650) of human TCERG1. This design may enable recognition of total TCERG1 in denaturing and native conditions; validate reactivity in your assay system.
• For Western blot, a band near 124 kDa is expected. Use appropriate positive (e.g., human cell lines known to express TCERG1) and negative controls.
• For ELISA, consider direct or capture-based formats; peptide competition assays may confirm specificity.
• Optimize antibody dilution and incubation conditions empirically, as polyclonal antibodies can exhibit lot-to-lot variability.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, offering researchers and manufacturers streamlined access to high-quality antibodies and ancillary reagents. This TCERG1 polyclonal antibody supports transcription and viral research applications. Our supply portfolio includes validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and sourcing of rare-target raw materials to accelerate your assay development and procurement workflows.