Anti-TCEB2 Rabbit Monoclonal Antibody for WB and ELISA - Q15370

Core Product Specifications and Parameters

Biological Background: TCEB2 Function and Localization

• TCEB2 (elongin-B, SIII) is the 18 kDa subunit of the elongin/SIII transcription elongation factor that increases RNA polymerase II processivity past template-encoded arrest sites. Related references: PMID:7638163
• As a core component of multiple cullin-2/5-RING E3 ubiquitin-protein ligase complexes (ECS), TCEB2 links substrate-recognition subunits (e.g., VHL, SOCS box proteins) to the ubiquitination machinery, mediating target protein ubiquitination and degradation. Related references: PMID:10205047, PMID:12004076, PMID:12050673
• Multiple ECS complexes are involved in the DesCEND pathway, recognizing C-end degrons for ubiquitination and degradation, as well as in specific processes like replisome disassembly, focal adhesion regulation, and histone methylation control. Related references: PMID:26138980, PMID:29775578, PMID:29779948, PMID:33268465, PMID:19920177, PMID:35512830, PMID:40440427, PMID:40963025
• During HIV-1 infection, TCEB2 is hijacked as part of an ECS complex that ubiquitinates and degrades antiviral APOBEC3 proteins. Related references: PMID:18562529, PMID:20532212, PMID:22190037, PMID:37640699
• TCEB2 is localized in the nucleus and undergoes post-translational modifications including acetylation and phosphorylation.

Experimental Guidance and Technical Tips

• The immunogen used is a recombinant fusion protein corresponding to amino acids 1-100 of human TCEB2. This N-terminal region is highly conserved, contributing to the antibody's cross-reactivity with mouse and rat.
• For Western blotting (WB), consider detecting a band at approximately 13 kDa. Validate the antibody's specificity in your sample system.
• For ELISA, optimize coating conditions and antibody dilutions according to standard protocols; the antibody can be used as a capture or detection antibody after appropriate pairing validation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutions with one-stop access to high-quality IVD raw materials, including validated antibody pairs, optimized monoclonal antibodies, and bulk ancillary reagents. This anti-TCEB2 antibody supports research in transcription elongation and ubiquitin-proteasome pathways, offering reliable performance for assay development. Tailored sourcing solutions for rare targets and technical consulting are available to streamline your pipeline from concept to clinic.