Anti-TCEB1 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q15369

Core Product Specifications and Parameters

Biological Background: Elongin-C (TCEB1) Function and Localization

• Elongin-C (TCEB1), also known as SIII p15, is the 15 kDa subunit of the general transcription elongation factor SIII, which increases RNA polymerase II transcription elongation past template-encoded arresting sites. Related references: PMID:7821821
• Elongin-C serves as a core component of multiple cullin-RING-based ECS (ElonginB/C-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complexes, mediating ubiquitination and degradation of diverse targets, including CKB, SUV39H1, CDO1, POLR2A, and MCM7. Related references: PMID:10205047, PMID:12004076, PMID:12050673, PMID:26138980, PMID:29775578, PMID:29779948, PMID:33268465, PMID:19920177, PMID:35512830, PMID:40440427, PMID:40963025
• During HIV-1 infection, Elongin-C is hijacked by viral Vif protein to form an ECS complex that ubiquitinates and degrades host antiviral factors APOBEC3F and APOBEC3G. Related references: PMID:18562529, PMID:20532212, PMID:22190037, PMID:37640699
• The protein is primarily localized to the nucleus, consistent with its roles in transcription and ubiquitination.
• TCEB1 is overexpressed in certain cancer cell lines, including prostate cancer PC-3 and breast cancer SK-BR-3, suggesting a potential role in oncogenesis.
• The gene undergoes alternative splicing and is involved in host-virus interactions and ubiquitin conjugation pathways.

Experimental Guidance and Technical Tips

• For Western blotting, a band at approximately 12 kDa is expected; validate with appropriate positive (e.g., HeLa, HEK293) and negative controls in your sample system.
• In immunohistochemistry (IHC-P), consider antigen retrieval methods and use cancer tissue sections (such as prostate or breast) as potential positive controls given the overexpression pattern.
• As a polyclonal antibody, it may detect multiple isoforms or degradation products; verify specificity using knockdown or knockout samples if available.
• The antibody cross-reacts with mouse and rat, enabling cross-species studies; optimize dilutions for each species and application.
• For ELISA, consider pairing with a matched detection antibody and validate using recombinant TCEB1 protein.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers and researchers with high-quality IVD raw materials, including validated antibodies like this anti-TCEB1 polyclonal antibody. We offer one-stop access to transcription and ubiquitination research reagents, from bulk ancillary buffers to custom rare-target development. Partner with us to streamline your assay development journey from concept to clinic.