Anti-TCEA1 Polyclonal Antibody for WB, ELISA - P23193

Core Product Specifications and Parameters

Biological Background: TCEA1 Function and Localization

• Protein Identity: TCEA1 encodes transcription elongation factor A protein 1, also designated transcription elongation factor S-II protein 1 or TFIIS.o, a member of the transcription elongation factor S-II family.
• Transcriptional Elongation Rescue: It is necessary for efficient RNA polymerase II transcription elongation past template-encoded arresting sites. These sites trap elongating polymerases in locked ternary complexes; S-II promotes cleavage of the nascent transcript, enabling elongation to resume from the new 3′-terminus.
• Subcellular Localization: The protein is predominantly localized to the nucleus, consistent with its function in transcription.
• Post-Translational Modifications: TCEA1 undergoes acetylation, phosphorylation, and ubiquitination (isopeptide bond formation), suggesting multiple layers of regulatory control.
• Structural Features: It contains zinc-finger motifs and binds DNA, which are critical for its role in transcription elongation and interaction with the transcription machinery.
• Alternative Splicing: Multiple isoforms are generated through alternative splicing, potentially contributing to functional diversity.
• Genomic Implications: The protein is associated with chromosomal rearrangement events, hinting at a role in genome stability or disease-related translocations.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 75-140 of human TCEA1, a region conserved within the S-II family. Cross-reactivity with other S-II proteins (e.g., TCEA2, TCEA3) should be assessed if needed.
• For Western blotting, a band around 34 kDa is expected. Optimize blocking and antibody dilution using appropriate positive controls (e.g., HeLa or HEK293 nuclear extracts).
• In ELISA, consider a direct or indirect format with recombinant TCEA1 protein or cell lysates; validate with known TFIIS-deficient samples.
• As a polyclonal antibody, lot-to-lot consistency may vary; include a reference standard in each experiment.
• The antibody is shipped on ice bags; store at −20°C upon receipt and avoid repeated freeze–thaw cycles.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio partners with diagnostic manufacturers, laboratories, and research institutes to provide comprehensive access to IVD raw materials and technical services. For transcription and gene regulation research, we offer validated antibody pairs, optimized monoclonal and polyclonal antibodies like this anti-TCEA1 reagent, and bulk ancillary reagents. Whether you need rare target sourcing or customized assay development support, our one-stop platform streamlines your transition from concept to clinical application.