Anti-TCEA1 Monoclonal Antibody for WB, IF/ICC, ELISA - P23193

Core Product Specifications and Parameters

Biological Background: TCEA1 Function and Localization

• TCEA1 (Gene ID: 6917) encodes transcription elongation factor A protein 1, also known as transcription elongation factor S-II protein 1 (TFIIS) and TFIIS.o.
• The protein localizes to the nucleus and plays a critical role in RNA polymerase II transcription elongation.
• It enables RNA polymerase II to read through template-encoded arrest sites by inducing cleavage of the nascent RNA transcript, thereby generating a new 3'-terminus for extension.
• TCEA1 belongs to the transcription elongation factor A family and contains a zinc-finger domain essential for its function.
• Post-translational modifications include acetylation, phosphorylation, isopeptide bond formation, and ubiquitination.
• The gene undergoes alternative splicing and has been reported to participate in chromosomal rearrangements.

Experimental Guidance and Technical Tips

• The antibody was raised against a synthetic peptide spanning amino acids 50–150 of human TCEA1; this epitope is conserved in mouse and rat, consistent with observed cross-reactivity.
• For Western blotting, nuclear extracts or whole-cell lysates are recommended; a specific band at approximately 34 kDa is expected.
• In immunofluorescence/ICC, TCEA1 shows a predominantly nuclear localization pattern; appropriate fixation and permeabilization should be optimized.
• ELISA applications may be used for quantitative detection of TCEA1 in various sample types; titrate the antibody to determine optimal working concentration.
• Although the antibody is listed for human, mouse, and rat, performance in other species should be empirically validated.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with a comprehensive portfolio of IVD raw materials. Our TCEA1 monoclonal antibody supports transcription and gene regulation research, and we offer end-to-end supply solutions including validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare-target raw materials. Partner with CamelBio to bridge your concept-to-clinic journey with reliable, high-quality raw material supply.