Anti-TCAP Rabbit Polyclonal Antibody for WB, ELISA - O15273

Core Product Specifications and Parameters

Biological Background: Telethonin Function and Localization

• Telethonin (also known as titin cap protein) is a muscle-specific protein encoded by the TCAP gene, primarily expressed in heart and skeletal muscle.
• It acts as a muscle assembly regulating factor, mediating the antiparallel assembly of titin (TTN) molecules at the Z-disk of the sarcomere, which is critical for sarcomere integrity and muscle contraction.
• Telethonin is a component of the sarcomeric Z-disk, where it interacts with the N-terminus of titin, forming a stable complex that serves as a structural template for myofibril organization.
• Subcellular localization is restricted to the cytoplasm, specifically at the myofibril sarcomere, consistent with its role in striated muscle ultrastructure.
• Mutations in the TCAP gene are associated with various muscle disorders, including limb-girdle muscular dystrophy type 2G (LGMD2G) and dilated cardiomyopathy (CMD1N), highlighting its clinical relevance.
• Telethonin undergoes phosphorylation, which may regulate its interaction with titin and other Z-disk proteins, influencing sarcomere dynamics.

Experimental Guidance and Technical Tips

• The immunogen used is a recombinant protein corresponding to the N-terminal region (amino acids 1-167) of human TCAP, which may facilitate recognition of the full-length protein in denaturing conditions.
• For Western blotting, typical primary antibody dilutions range from 1:500 to 1:2000; however, optimal dilution should be determined experimentally using the relevant sample matrix.
• The antibody has been validated for ELISA; appropriate coating and blocking conditions should be optimized based on the specific assay format.
• Given the polyclonal nature, cross-reactivity with other titin-binding proteins or isoforms in some species cannot be excluded; specificity should be confirmed in the intended sample system.
• Tissue specificity is restricted to heart and skeletal muscle; therefore, using positive control lysates from these tissues is recommended for WB validation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop sourcing partner for IVD raw materials, supporting diagnostic manufacturers from concept to clinic. We offer this high-quality anti-TCAP polyclonal antibody alongside a comprehensive portfolio of validated antibody pairs, bulk reagents, and rare target raw materials. Whether you're developing assays for muscle disease markers or exploring sarcomere biology, rely on CamelBio's integrated supply chain to accelerate your next development milestone.