Anti-TCAP Rabbit Monoclonal Antibody for WB, IF-P, ELISA - O15273

Core Product Specifications and Parameters

Biological Background: Telethonin (TCAP) Function and Localization

• Telethonin (also known as titin cap protein) is a sarcomeric protein encoded by the TCAP gene in humans.
• It functions as a muscle assembly regulating factor, mediating the antiparallel assembly of titin molecules at the Z-disk.
• Telethonin is specifically expressed in heart and skeletal muscle.
• Subcellularly, it localizes to the cytoplasm, myofibril, and sarcomere.
• Mutations in TCAP are associated with cardiomyopathy (CMD1N, CMH25) and limb-girdle muscular dystrophy (LGMD2G, LGMDR7), highlighting its role in muscle disease.
• Post-translational modifications include phosphorylation, as indicated by the Phosphoprotein keyword.
• The protein has a molecular weight of approximately 19kDa, consistent with the observed 19kDa band in western blotting.
• The 3D structure of telethonin has been determined, providing insights into its interaction with titin.

Experimental Guidance and Technical Tips

• For Western blotting (WB), use this antibody against lysates from human, mouse, or rat cardiac or skeletal muscle tissues to detect the 19kDa telethonin band.
• In immunofluorescence (IF-P), validate the antibody in fixed sarcomere preparations; ensure appropriate permeabilization to access cytoplasmic targets.
• For ELISA, this antibody, raised against recombinant protein, may perform well; however, validate in the relevant sample system.
• Given the broad cross-reactivity with human, mouse, and rat, the antibody is suitable for translational research across these species.
• The immunogen covers the N-terminal domain (aa 1-167), which includes critical residues for titin binding; thus, the antibody may recognize functional epitopes.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to high-quality IVD raw materials for skeletal and cardiac muscle research. Our portfolio includes validated antibody pairs, optimized monoclonal antibodies like this anti-TCAP clone, bulk ancillary reagents, and rare target raw-material sourcing. From concept to clinic, we streamline your development with integrated technical services and consulting.