Anti-TCAP Polyclonal Antibody for WB, ELISA - O15273

Core Product Specifications and Parameters

Biological Background: Telethonin Function and Localization

• Telethonin (also known as titin cap protein) is encoded by the TCAP gene and is predominantly expressed in heart and skeletal muscle.
• It serves as a muscle assembly regulator, mediating the antiparallel assembly of titin (TTN) molecules at the sarcomeric Z-disk.
• Subcellularly, telethonin localizes to the cytoplasm, myofibrils, and sarcomeres.
• Mutations in TCAP are associated with cardiomyopathies and limb-girdle muscular dystrophy type 2G (LGMD2G/LGMDR7).
• The protein is subject to phosphorylation and has a molecular weight of approximately 19 kDa.
• Additional features include known 3D structures and identification in proteomics studies.

Experimental Guidance and Technical Tips

• For Western blot analysis, consider using heart or skeletal muscle tissue lysates as positive controls, expecting a band near 19 kDa.
• When performing ELISA, validate antibody binding with recombinant TCAP protein or native samples.
• The antibody cross-reacts with human, mouse, and rat TCAP; therefore, it may be suitable for multi-species comparative studies.
• Optimize antibody dilution and incubation conditions in your specific experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target procurement. For research into sarcomere assembly and muscle-related disorders, our TCAP polyclonal antibody provides a reliable reagent for Western blot and ELISA applications. Whether you need additional muscle-specific biomarkers or technical consultation, we are here to support your project from concept to clinic.