Anti-TC10/RHOQ Monoclonal Antibody for WB, IHC-P, ELISA - P17081

Core Product Specifications and Parameters

Biological Background: TC10/RHOQ Function and Localization

• Rho-related GTP-binding protein RhoQ, also known as Ras-like protein TC10 or Ras-like protein family member 7A (gene RHOQ/ARHQ/RASL7A/TC10), is a member of the Rho family of small GTPases.
• It acts as a molecular switch by cycling between active GTP-bound and inactive GDP-bound states, and in its active form binds to various effector proteins to regulate cellular responses.
• Specifically, TC10/RHOQ induces the formation of thin, actin-rich surface projections called filopodia, contributing to cytoskeletal reorganization.
• It plays a role in epithelial cell polarization processes, likely through its involvement in directed membrane trafficking.
• Additionally, TC10/RHOQ may facilitate CFTR (cystic fibrosis transmembrane conductance regulator) trafficking to the plasma membrane.
• It is localized to the cytoplasm and cell membrane, consistent with its role as a plasma membrane-associated small GTPase.
• The protein undergoes lipid modification (prenylation) and methylation, and features GTP-binding, hydrolase activity, and metal-binding (magnesium) properties.

Experimental Guidance and Technical Tips

• Consider that the immunogen consists of a recombinant fusion protein corresponding to amino acids 100-205 of human TC10/RHOQ; this region may be critical for specific detection.
• For Western blot (WB), a band around 23 kDa is expected; validate in relevant human, mouse, or rat cell lysates.
• In immunohistochemistry (IHC), optimize antigen retrieval and antibody dilution using known positive tissue controls.
• For ELISA, titrate the antibody against purified recombinant protein or cell extracts to establish working concentrations.
• Note that the antibody is monoclonal, ensuring batch-to-batch consistency.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research institutions with comprehensive access to high-quality IVD raw materials. This anti-TC10/RHOQ monoclonal antibody exemplifies our offering of optimized reagents for cell biology, cytoskeleton, and membrane trafficking studies. From validated antibody pairs to rare target sourcing, CamelBio streamlines your development from concept to clinic.