Anti-TBX6 Rabbit Polyclonal Antibody for WB, ELISA - O95947

Core Product Specifications and Parameters

Biological Background: TBX6 Function and Localization

• T-box transcription factor TBX6, encoded by the TBX6 gene, is a member of the T-box family of DNA-binding proteins and undergoes alternative splicing.
• Plays an essential role in the determination of axial stem cell fate, promoting mesodermal differentiation while inhibiting neural development by down-regulating the N1 enhancer of SOX2 (By similarity).
• Involved in left/right axis determination during embryogenesis, acting through effects on Notch signaling around the node and influencing nodal cilia morphology and motility (By similarity).
• Subcellular localization is predominantly nuclear, consistent with its function as a transcription factor.
• Expressed in fetal tissues including tail bud, posterior spinal tissue, intervertebral disk, and testis; in adults, detected in testis, kidney, lung, muscle, and thymus.
• Associated with diseases such as dwarfism (keyword), and its activity is regulated by post-translational modifications including ubiquitination (Ubl conjugation).
• Annotated with keywords: Alternative splicing, Developmental protein, DNA-binding, Nucleus, Transcription, Transcription regulation, Reference proteome.

Experimental Guidance and Technical Tips

• The immunogen comprises amino acids 1-280 of human TBX6; consider validating reactivity against isoforms or related T-box family members if cross-reactivity is a concern.
• For Western blot, use standard SDS-PAGE and transfer conditions; molecular weight approximately 47 kDa.
• ELISA applications may require optimization of coating and detection conditions depending on the assay format and sample type.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with a one-stop supply of IVD raw materials, including validated monoclonal and polyclonal antibodies like this TBX6 rabbit pAb. Whether you need optimized antibody pairs, bulk quantities, or custom reagent sourcing for developmental biology or rare disease research, our integrated supply chain accelerates your transition from concept to clinic.