Anti-TBX19 Rabbit Polyclonal Antibody for WB, ELISA - O60806

Core Product Specifications and Parameters

Biological Background: TBX19 Function and Localization

• TBX19 (also known as TPIT) is a T-box transcription factor that belongs to the T-box family of developmental regulators.
• It functions as both a transcriptional activator and repressor, depending on the target gene context.
• Specifically, TBX19 activates pro-opiomelanocortin (POMC) gene expression, which is critical for pituitary hormone production.
• It represses the promoters of the alpha glycoprotein subunit and thyroid-stimulating hormone beta (TSHB), highlighting its role in pituitary cell lineage specification.
• The protein is localized to the nucleus, consistent with its role as a DNA-binding transcription factor.
• TBX19 contains a T-box DNA-binding domain that mediates sequence-specific DNA recognition to regulate target genes.
• Mutations in TBX19 are associated with isolated adrenocorticotropic hormone (ACTH) deficiency, reflecting its clinical significance in pituitary disorders.
• The protein is part of the reference proteome and has been identified in proteomics studies.

Experimental Guidance and Technical Tips

• The immunogen covers the C-terminal region (aa 229–448) of human TBX19; consider using whole cell lysates from pituitary-derived cell lines or other relevant human samples for Western blot detection.
• For ELISA, the antibody can be tested in both direct and sandwich formats; optimize coating concentrations and detection conditions experimentally.
• As a polyclonal preparation, verify lot-to-lot consistency and include appropriate positive and negative controls in each experiment.
• Standard blocking (e.g., 5% non-fat milk or BSA) and washing protocols are recommended; adjust incubation times and temperatures based on sample and assay requirements.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with streamlined access to high-quality IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies like this TBX19 pAb, and bulk ancillary reagents. Our one-stop platform bridges the gap from concept to clinic, supporting rare target sourcing and accelerating developmental biology and pituitary disorder research with reliable, scalable supply solutions.