Anti-TBRG4 Polyclonal Antibody for WB - Q969Z0

Core Product Specifications and Parameters

Biological Background: TBRG4 Function and Localization

• TBRG4 (FAST kinase domain-containing protein 4) is also known as CPR2, FASTKD4, and transforming growth factor beta regulator 4, playing a crucial role in mitochondrial RNA processing.
• It stabilizes a subset of mature mitochondrial RNA species, including MT-CO1, MT-CO2, MT-CYB, MT-CO3, MT-ND3, MT-ND5, and MT-ATP8/6, ensuring proper mitochondrial gene expression.
• TBRG4 may contribute to cell cycle progression, as suggested by functional studies that highlight its dual involvement in mitochondrial homeostasis and cellular proliferation. Related references: PMID:9383053
• The protein localizes to the mitochondrial matrix, consistent with its role in post-transcriptional regulation of mitochondrial RNAs.
• TBRG4 is ubiquitously expressed, with highest levels observed in smooth muscle, and can be detected in tissues such as spleen, thymus, testis, ovary, colon, heart, kidney, brain, lung, liver, and white adipose tissue.
• Keywords associated with TBRG4 include alternative splicing, phosphoprotein, transit peptide, and proteomics identification, reflecting its regulated expression and mitochondrial targeting.
• As a member of the FAST kinase family, TBRG4 shares structural domains that mediate RNA binding and processing, making it a valuable target for studying mitochondrial disorders and cell cycle regulation.

Experimental Guidance and Technical Tips

• For Western blot analysis, expect a band at approximately 71 kDa; optimize lysate preparation and loading controls for mitochondrial proteins.
• In immunofluorescence/ICC, due to mitochondrial matrix localization, consider using mild permeabilization and co-staining with mitochondrial markers such as TOM20 or COXIV to confirm specific staining.
• When performing ELISA, validate coating conditions and antibody pairing; note that polyclonal antibodies may exhibit lot-to-lot variability, so include appropriate negative controls and standardize protocols.
• Cross-reactivity with mouse and rat (human immunogen sequence 382-631) suggests the antibody may recognize conserved epitopes; verify with species-specific positive controls in initial experiments.
• Since the immunogen covers a specific internal region, confirm reactivity in your target sample system using known positive and negative cell lines.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with comprehensive access to IVD raw materials, including validated antibody pairs and optimized polyclonal antibodies like this anti-TBRG4 product. Our portfolio spans mitochondrial and cell cycle targets, supported by bulk ancillary reagents and custom sourcing for rare targets. With a focus on bridging discovery and clinical application, we help streamline your assay development from concept to clinic.