Anti-TBR1 Monoclonal Antibody for WB, IF-P, ELISA - Q16650

Core Product Specifications and Parameters

Biological Background: TBR1 Function and Localization

• TBR1 (T-box brain protein 1, also known as TES-56) is a member of the T-box family of transcription factors.
• Acts as a transcriptional repressor critical for cerebral cortex development, including neuronal migration and arealization (PubMed:25232744, PubMed:30250039). Related references: PMID:25232744 PMID:30250039
• Represses FEZF2 to confine corticospinal tract origin to layer 5 neurons (By similarity).
• Localizes to the nucleus.
• Highly expressed in the brain.
• Associated with neurodevelopmental disorders such as autism spectrum disorder and intellectual disability.
• Undergoes alternative splicing and phosphorylation.

Experimental Guidance and Technical Tips

• The immunogen used is a synthetic peptide spanning amino acids 1-100 of human TBR1. The antibody may recognize full-length TBR1 and potentially N-terminal isoforms or cleavage products. Consider this when interpreting band patterns.
• For Western blot (WB), TBR1 migrates at approximately 74 kDa. Use nuclear-enriched lysates from brain tissue or neuronal cell lines (e.g., SH-SY5Y) for optimal detection. Validate with a known positive control.
• In immunofluorescence (IF-P), TBR1 localizes to the nucleus. Adequate permeabilization and fixation are essential. Test different conditions to achieve specific staining.
• The antibody cross-reacts with mouse TBR1, making it suitable for studies using mouse cortical development models.
• For ELISA, include appropriate negative and positive controls. The peptide immunogen can be used as a positive control for assay validation.
• As with all antibody-based applications, empirical optimization of dilution and blocking conditions is recommended for each sample type.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers diagnostic manufacturers and research labs with reliable one-stop access to IVD raw materials for neuroscience and developmental disorder targets. We supply validated monoclonal antibodies, antibody pairs, and ancillary reagents for T-box family proteins like TBR1. Whether you need bulk reagents or custom sourcing for niche targets, our team provides seamless procurement support from concept to clinic.