Anti-TBP Rabbit Polyclonal Antibody for WB and ELISA - P20226

Core Product Specifications and Parameters

Biological Background: TBP Function and Localization

• TBP (TATA-box-binding protein) is a general transcription factor that plays a central role in initiating RNA polymerase II-dependent transcription. It functions as a subunit of the TFIID complex. Related references: PMID:33795473
• TBP specifically binds to TATA-box promoter elements, bending DNA and facilitating assembly of the pre-initiation complex (PIC), which includes RNA polymerase II and other general transcription factors. Related references: PMID:2194289, PMID:2363050, PMID:2374612
• It is a component of TFIID, a large complex comprising TBP and multiple TATA-binding protein-associated factors (TAFs TAF1–TAF13). Within TFIID, TBP forms the TFIID-A module together with TAF3 and TAF5. Related references: PMID:27007846, PMID:33795473
• TBP also participates in RNA polymerase III transcription via a BRF2-containing complex, and in RNA polymerase I transcription as part of the SL1/TIF-IB complex. Related references: PMID:26638071, PMID:15970593
• The protein localizes exclusively to the nucleus, where it engages with promoter DNA to regulate gene expression.
• TBP is widely expressed across tissues, with highest expression levels found in testis and ovary, reflecting its essential role in global transcription.
• Alternative splicing of the TBP gene yields multiple isoforms, and mutations in TBP are associated with neurodegenerative diseases such as spinocerebellar ataxia type 17 (SCA17).

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide corresponding to the N-terminal region (amino acids 1–100) of human TBP. Researchers should consider that different isoforms or post-translational modifications may affect antibody binding; validation in the specific experimental system is recommended.
• For Western blot, the predicted molecular weight of TBP is ~38 kDa. Multiple bands or slight shifts may be observed due to alternative splicing or modifications; include appropriate controls.
• The antibody is reported to cross-react with mouse and rat TBP, making it suitable for studies across these species. However, confirm cross-reactivity in your model by testing with known positive samples.
• For ELISA applications, optimize antibody concentration and assay conditions. Note that polyclonal antibodies may exhibit higher background; negative controls are essential.
• This antibody is supplied as a polyclonal antibody from rabbit, which typically recognizes multiple epitopes. This can be advantageous for denaturing assays like WB but may require careful blocking and washing steps.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a one-stop sourcing solution for high-quality IVD raw materials, including this anti-TBP polyclonal antibody for transcription research. Our portfolio also includes validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents to accelerate your diagnostic assay development. For rare or custom targets, our global supply network ensures reliable access to critical raw materials from concept to clinic.