Anti-TBL2 Rabbit Polyclonal Antibody for WB and ELISA - Q9Y4P3

Core Product Specifications and Parameters

Biological Background: TBL2 Function and Localization

• Transducin beta-like protein 2 (TBL2), also designated WS-betaTRP or WBSCR13, is a human protein encoded by the TBL2 gene. It belongs to the WD repeat protein family.
• TBL2 contains multiple WD repeat domains, which commonly serve as scaffolds for protein–protein interactions, suggesting its involvement in multiprotein complex assembly or signal transduction.
• According to UniProt annotations, TBL2 is a phosphoprotein and can undergo isopeptide bond formation through ubiquitin-like (Ubl) conjugation, indicating post-translational regulation via phosphorylation and ubiquitin-related pathways.
• The TBL2 gene is located within the Williams-Beuren syndrome (WBS) critical region on chromosome 7q11.23; hemizygous deletion of this region, including TBL2, is linked to the developmental disorder.
• TBL2 has been detected in proteomic studies, confirming its expression in human tissues. Its predicted molecular weight is approximately 50 kDa.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 100–430 of human TBL2; for Western blot, expect a band at ~50 kDa. Verify band size using appropriate molecular weight markers and validate detection in lysates from recommended species (human, mouse, rat).
• As a polyclonal antibody, it may recognize multiple epitopes, providing robust signals but potentially varying between production lots. Consider titrating the antibody (e.g., 1:500–1:2000 for WB) to optimize signal-to-noise in your experimental system.
• For ELISA applications, the antibody can be used as a capture or detection reagent; indirect ELISA using recombinant TBL2 protein or peptide is a suitable starting point. Confirm performance with a standard curve.
• Cross-reactivity is predicted for human, mouse, and rat based on amino acid sequence similarity; however, always test reactivity in your target species and tissue type before large-scale experiments.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers comprehensive access to IVD raw materials and technical support, empowering research on WD repeat proteins and Williams-Beuren syndrome. We provide validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents to streamline your diagnostic development. Our one-stop sourcing platform bridges from early research to clinical application, facilitating rare target reagent procurement and technical consulting.