Anti-TBL1XR1 Polyclonal Antibody for WB, IF/ICC, ELISA - Q9BZK7

Core Product Specifications and Parameters

Biological Background: TBL1XR1 Function and Localization

• TBL1XR1 (Transducin beta-like 1X-related protein 1) is a F-box-like/WD repeat-containing protein, also known as TBLR1 or nuclear receptor corepressor/HDAC3 complex subunit TBLR1.
• It plays an essential role in transcription activation mediated by nuclear receptors, acting as an integral component of the N-Cor corepressor complex.
• TBL1XR1 facilitates the recruitment of the ubiquitin/19S proteasome complex to transcription units, leading to the proteasomal degradation of the N-Cor complex and allowing cofactor exchange for transcription initiation.
• The protein localizes to the nucleus, consistent with its function in transcriptional regulation.
• It is widely expressed in tissues including the pituitary, hypothalamus, white and brown adipose tissue, muscle, and liver.
• Keywords from UniProt include chromatin regulator, transcription activator/repressor, and Ubl conjugation pathway, reflecting its roles in both gene activation and repression.
• TBL1XR1 undergoes post-translational modifications such as acetylation and phosphorylation, which may modulate its activity.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 120–170 of human TBL1XR1, a region that is conserved in mouse, supporting the observed human and mouse cross-reactivity. Users should verify reactivity in other species as needed.
• For Western blot, a band at approximately 56 kDa (predicted molecular weight) is expected. Consider using appropriate loading controls and validating expression in the cell or tissue type of interest.
• For immunofluorescence/immunocytochemistry (IF/ICC), nuclear staining is anticipated given the protein's subcellular localization. Optimize fixation and permeabilization conditions for your sample.
• For ELISA, the antibody can be used for detection; titrate the antibody and validate against appropriate positive and negative controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, laboratories, and research institutes a one-stop solution for IVD raw materials, technical services, and consulting from concept to clinic. Our portfolio includes validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing. This anti-TBL1XR1 polyclonal antibody supports research into nuclear receptor-mediated transcription and ubiquitin-proteasome pathways, with applications in WB, IF/ICC, and ELISA.