Anti-TBCE Polyclonal Antibody for IF-P and ELISA - Q15813

Core Product Specifications and Parameters

Biological Background: TBCE Function and Localization

• TBCE, also known as tubulin-specific chaperone E or tubulin-folding cofactor E, is a key player in the tubulin folding pathway.
• It participates in the second step of tubulin heterodimer assembly and regulates tubulin heterodimer dissociation.
• TBCE is essential for proper organization of the microtubule cytoskeleton and formation of the mitotic spindle.
• It is particularly important for maintaining the neuronal microtubule network, highlighting its role in neural function.
• The protein is localized to the cytoplasm and associates with the cytoskeleton.
• TBCE undergoes post-translational modifications such as acetylation and phosphorylation, which may regulate its function.
• The TBCE gene undergoes alternative splicing, potentially yielding isoforms with diverse roles.
• Mutations in TBCE are associated with diseases including dwarfism, intellectual disability, and neurodegeneration, underscoring its clinical relevance.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant fusion protein spanning amino acids 328–527 of human TBCE; the antibody may detect both native and denatured forms under appropriate conditions.
• For immunofluorescence-paraffin (IF-P), consider optimizing antigen retrieval steps as the epitope may be masked in formalin-fixed tissues.
• In ELISA applications, the antibody can be used to capture or detect TBCE; verify linearity and sensitivity with known standards.
• Cross-reactivity with mouse TBCE allows for use in mouse model studies; however, confirm species-specific reactivity in your experimental system.
• Include proper controls, such as knockout cell lines or blocking peptides, to ensure signal specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutes with a streamlined sourcing solution for IVD raw materials. For microtubule biology and tubulin folding research, we offer a portfolio of validated antibodies, including this anti-TBCE polyclonal antibody, plus bulk buffers and ancillary reagents to support your assay development. Our team also assists with rare target sourcing to bridge gaps in your workflow.