Anti-TBCCD1 Polyclonal Antibody for WB, IHC-P, ELISA - Q9NVR7

Core Product Specifications and Parameters

Biological Background: TBCCD1 Function and Localization

• TBCCD1 (TBCC domain-containing protein 1) is a member of the TBCC family, characterized by the presence of a tubulin binding cofactor C domain.
• It plays a role in the regulation of centrosome and Golgi apparatus positioning, which influences cell shape and migration.
• The protein localizes predominantly to the centrosome and spindle pole within the cytoplasm, consistent with its role in microtubule organization.
• Alternative splicing of the TBCCD1 gene generates multiple isoforms, potentially contributing to functional diversity.
• As a component of the cytoskeleton-associated proteome, TBCCD1 is involved in maintaining cell polarity and motility.
• It has been identified through large-scale proteomics studies as part of the human reference proteome.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide from the C-terminal region (amino acids 456–557) of human TBCCD1; this design may confer specificity for isoforms retaining this sequence.
• For Western blot, consider starting with a dilution range recommended for rabbit polyclonal antibodies and adjust based on signal intensity in your sample system.
• In immunohistochemistry (paraffin-embedded sections), antigen retrieval conditions may need optimization; perform a dilution series to determine optimal staining.
• Cross-reactivity with mouse and rat is reported; validate species-specific expression patterns using appropriate positive and negative controls.
• ELISA can be used for titer determination or quantitative analysis; ensure the coating antigen matches the immunogen region for best sensitivity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio stands as a dependable one-stop partner for IVD raw-material sourcing, offering validated antibody pairs, bulk ancillary reagents, and access to rare targets. This anti-TBCCD1 polyclonal antibody supports research into centrosome biology, cell migration, and cytoskeletal organization. Leverage CamelBio’s integrated supply chain to streamline procurement from discovery to clinical application, backed by expert technical consultation.