Anti-TBCC Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q15814

Core Product Specifications and Parameters

Biological Background: TBCC Function and Localization

• TBCC (tubulin-specific chaperone C), also known as tubulin-folding cofactor C (CFC), operates in the cytoplasm.
• It mediates the final step of the tubulin folding pathway, which is essential for the formation of functional α/β-tubulin heterodimers.
• The protein displays a high level of expression in the retina, particularly in the rod and cone photoreceptor cells.
• At the subcellular level, TBCC is strongly localized to the photoreceptor connecting cilium, a structure critical for sensory function.
• Post-translational modifications such as phosphorylation and acetylation have been detected on TBCC, as indicated by proteomics analyses.
• The protein belongs to the TBCC family, with a calculated molecular weight of approximately 39 kDa.

Experimental Guidance and Technical Tips

• For Western blot, expect a specific band at approximately 39 kDa; confirm with appropriate positive control lysates (e.g., retinal tissue or cell lines expressing TBCC).
• In immunofluorescence/ICC, TBCC exhibits cytoplasmic staining with enrichment at the connecting cilium in photoreceptors; adjust fixation and permeabilization protocols to preserve this localization.
• The antibody is compatible with ELISA; consider pairing with a detection antibody or using it in a capture format after adequate titration.
• Validate antibody specificity in your experimental system by using TBCC knockdown or knockout controls to confirm signal reduction.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research institutes a streamlined sourcing platform for premium IVD raw materials, including this anti-TBCC polyclonal antibody for investigations into tubulin dynamics and retinal biology. Our one-stop supply chain covers validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets—empowering your assay development from concept to clinic.