Anti-TBCB Polyclonal Antibody for WB, IHC-P, ELISA - Q99426

Core Product Specifications and Parameters

Biological Background: TBCB Function and Localization

• TBCB (tubulin-folding cofactor B), also known as cytoskeleton-associated protein 1 (CKAP1), is a chaperone that binds alpha-tubulin folding intermediates after their release from the cytosolic chaperonin, facilitating proper tubulin heterodimer formation. Related references: PMID:9265649
• It plays a critical role in the regulation of microtubule dynamics by controlling tubulin heterodimer dissociation.
• The protein may act as a negative regulator of axonal growth, implicating it in neurogenesis and developmental processes.
• TBCB localizes primarily to the cytoplasm and associates with the cytoskeleton, consistent with its role in tubulin biogenesis and microtubule network organization.
• It is ubiquitously expressed across most human tissues, reflecting its fundamental role in tubulin biology.
• Post-translational modifications include acetylation, phosphorylation, and ubiquitination, which may modulate its chaperone activity and interactions.
• Genetic variants in TBCB have been linked to autism spectrum disorder and intellectual disability, highlighting its importance in neuronal development.

Experimental Guidance and Technical Tips

• As the immunogen comprises the full-length human TBCB protein (aa 1-244), the resulting polyclonal antibody likely recognizes multiple epitopes, providing robust signal in denaturing western blots. Consider using appropriate loading controls and validating in relevant cell lines.
• For immunohistochemistry (paraffin), antigen retrieval steps should be optimized, and staining validated in known positive tissues.
• In ELISA applications, the antibody can serve as a capture or detection reagent, but pairing with a suitable secondary or matched antibody pair is advised for best performance.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive suite of IVD raw materials, including this anti-TBCB polyclonal antibody for research into cytoskeletal dynamics, tubulin folding, and neurodevelopmental disorders. Our integrated supply chain provides validated antibody pairs, bulk ancillary reagents, and tailored sourcing for rare targets, empowering your assay development from concept to clinic.