Anti-TBC1D4 Polyclonal Antibody for WB, IF/ICC, ELISA - O60343

Core Product Specifications and Parameters

Biological Background: TBC1D4 Function and Localization

• TBC1D4 (TBC1 domain family member 4), also known as AS160 (Akt substrate of 160 kDa), is a GTPase-activating protein (GAP) that regulates small Rab GTPases, including RAB2A, RAB8A, RAB10, and RAB14, controlling intracellular vesicle trafficking.
• Isoform 2 of TBC1D4 promotes insulin-induced translocation of the glucose transporter SLC2A4/GLUT4 to the plasma membrane, thereby enhancing glucose uptake—a critical process in metabolic regulation.
• TBC1D4 is widely expressed, with isoform 1 being highly expressed in skeletal muscle and heart, while isoform 2 shows strong expression in adrenal and thyroid glands, lung, kidney, colon, brain, and adipose tissue.
• At the protein level, TBC1D4 is expressed in pancreatic islets (including beta cells) and its expression is decreased twofold in type 2 diabetes patients, linking it to diabetes pathology.
• TBC1D4 is a cytoplasmic protein that undergoes multiple post-translational modifications, including phosphorylation, acetylation, and methylation, which modulate its activity and subcellular localization.
• Alternative splicing generates multiple isoforms of TBC1D4, contributing to its tissue-specific functional diversity.
• TBC1D4 is upregulated in T-cells from patients with atopic dermatitis, suggesting potential roles in immune function.

Experimental Guidance and Technical Tips

• For western blot, expect a band at approximately 147 kDa; consider using a high-molecular-weight protein ladder and optimized transfer conditions for large proteins.
• The immunogen sequence (aa 1059–1298) lies downstream of the known phospho-Akt substrate motif; verify if the antibody recognizes both phosphorylated and unphosphorylated forms based on your experimental needs.
• This antibody cross-reacts with mouse and rat TBC1D4; validate specificity in relevant tissue lysates (e.g., skeletal muscle, adipose tissue) and include appropriate controls.
• For immunofluorescence, cytoplasmic detection may require permeabilization with Triton X-100 or saponin; optimize fixation and blocking to preserve antigenicity and reduce background.
• In ELISA, use the recombinant immunogen as a positive control to establish standard curves and determine assay sensitivity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with comprehensive access to high-quality IVD raw materials. This TBC1D4 polyclonal antibody supports discovery in glucose metabolism and diabetes research. We offer validated antibody pairs, bulk ancillary reagents, and custom sourcing for rare targets—helping streamline your workflow from concept to clinic.