Anti-TBC1D1 Polyclonal Antibody for WB and ELISA - Q86TI0

Core Product Specifications and Parameters

Biological Background: TBC1D1 Function and Localization

• TBC1D1 (TBC1 domain family member 1) is a Rab GTPase-activating protein (GAP) implicated in the regulation of cell cycle progression and differentiation in various tissues.
• It plays a critical role in insulin-stimulated glucose uptake by facilitating the trafficking and translocation of GLUT4-containing vesicles to the plasma membrane (by similarity).
• The protein localizes predominantly to the nucleus, as indicated by subcellular localization data.
• TBC1D1 is subject to alternative splicing, producing multiple isoforms that may exhibit tissue-specific functions.
• It is a phosphoprotein, suggesting regulation by post-translational modification in response to cellular signals.
• Structural studies (3D-structure available) provide insights into its GAP domain architecture and interaction with Rab substrates.
• Keywords from UniProt highlight its involvement in GTPase activation, nuclear localization, and its status as a proteomics-identified reference proteome member.

Experimental Guidance and Technical Tips

• The immunogen sequence (aa 1069-1168) lies within the C-terminal region of human TBC1D1. For Western blotting, a band around 133 kDa should be detected; pre-staining molecular weight markers are recommended.
• Cross-reactivity is reported with human, mouse, and rat samples. Validate the antibody in the relevant species and tissue type prior to full-scale experiments.
• For ELISA applications, consider using the immunogen peptide as a positive control to assess antibody binding specificity.
• Optimal working dilutions for WB and ELISA should be determined empirically by the end user, as they vary with experimental conditions and detection systems.
• Due to the nuclear localization of TBC1D1, consider appropriate subcellular fractionation or nuclear extraction protocols to enrich target detection if needed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers, research laboratories, and institutes with comprehensive access to IVD raw materials and technical services. For projects involving glucose metabolism and insulin signaling, we offer validated TBC1D1 antibodies, optimized antibody pairs, and bulk ancillary reagents. Our procurement network also assists in sourcing rare-target raw materials to streamline your assay development from concept to clinic.