Anti-TAZ Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q9GZV5

Core Product Specifications and Parameters

Biological Background: TAZ Function and Localization

• Encoded by the WWTR1 gene (also known as TAZ), WW domain-containing transcription regulator protein 1 is a transcriptional coactivator with a PDZ-binding motif.
• Transcriptional coactivator acting as a downstream regulatory target in the Hippo signaling pathway, playing a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. Related references: PMID:11118213 PMID:18227151 PMID:23911299
• The Hippo pathway core kinase cascade involves STK3/MST2 and STK4/MST1 with SAV1 phosphorylating LATS1/2 with MOB1, subsequently phosphorylating and inactivating YAP1 and WWTR1/TAZ. Related references: PMID:18227151
• Enhances PAX8 and NKX2-1/TTF1-dependent gene activation. Related references: PMID:19010321
• In conjunction with YAP1, regulates TGFB1-dependent SMAD2 and SMAD3 nuclear accumulation and couples SMADs to the transcriptional machinery. Related references: PMID:18568018
• Regulates embryonic stem-cell self-renewal, promotes cell proliferation and epithelial-mesenchymal transition. Related references: PMID:18227151 PMID:18568018
• Subcellular localization includes nucleus, cytoplasm, cell membrane, and tight junctions.
• Highly expressed in kidney, heart, placenta, and lung; also expressed in thyroid tissue.
• Classified as a proto-oncogene; subject to phosphorylation and ubiquitination, with a coiled coil domain.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning amino acids 300–400 of human TAZ. Epitope recognition may be conformation-independent; consider its suitability for denaturing and native applications.
• For Western blot (WB), the predicted molecular weight is approximately 44 kDa. Use nuclear/cytoplasmic extracts as appropriate and validate with positive and negative controls.
• Immunofluorescence/immunocytochemistry (IF/ICC) may require optimized fixation and permeabilization to detect nuclear and cytoplasmic staining. Co-staining with tight junction markers can confirm localization.
• For ELISA, consider pairing with a capture antibody if developing a sandwich assay, or use direct coating of the immunogen peptide for performance screening.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with a comprehensive range of IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. For the TAZ target, our portfolio supports Hippo signaling and transcription regulation research with high-quality antibodies and technical consulting. From concept to clinic, we streamline procurement to accelerate your assay development and biomarker validation.