Anti-TAZ Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q9GZV5

Core Product Specifications and Parameters

Biological Background: TAZ Function and Localization

• WWTR1/TAZ is a transcriptional coactivator acting as a downstream effector of the Hippo signaling pathway, controlling organ size and tumor suppression by regulating proliferation and apoptosis. Related references: PMID:11118213 PMID:18227151 PMID:23911299

• TAZ enhances PAX8 and NKX2-1/TTF1-dependent gene activation and works with YAP1 to regulate TGFB1-dependent SMAD2/3 nuclear accumulation, coupling SMADs to the transcriptional machinery. Related references: PMID:19010321 PMID:18568018

• The protein plays a key role in embryonic stem-cell self-renewal, cell proliferation, and epithelial-mesenchymal transition (EMT). Related references: PMID:18227151 PMID:18568018

• It localizes to the nucleus, cytoplasm, cell membrane, and tight junctions, reflecting its dynamic shuttling and regulatory roles.

• TAZ is highly expressed in kidney, heart, placenta, lung, and thyroid tissue.

• The protein contains a WW domain, coiled-coil region, and a PDZ-binding motif, and is regulated by phosphorylation and ubiquitination (isopeptide bond). Keywords: Activator, Proto-oncogene, Transcription regulation, Ubl conjugation.

• It functions as a proto-oncogene; overexpression promotes tumorigenesis.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 1-230 of human TAZ, which includes the N-terminal TEAD-binding domain; thus the antibody may recognize both full-length and truncated forms.
• For Western blot (WB), a band around 44 kDa is expected in lysates from human, mouse, and rat cell lines or tissues; validate with appropriate positive controls (e.g., HEK293T, HeLa).
• In immunohistochemistry (IHC-P), antigen retrieval may be required; test both nuclear and cytoplasmic staining patterns depending on cell context.
• For immunofluorescence (IF/ICC), confirm nuclear and/or junctional localization; permeabilization is essential.
• ELISA performance depends on coating antigen and detection format; optimize for your specific assay.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic developers and research laboratories with reliable access to validated IVD raw materials, including high-quality primary antibodies, antigen pairs, and ancillary reagents. Our TAZ polyclonal antibody supports research into Hippo pathway signaling, cancer biology, and transcription regulation, offering robust performance across multiple applications. Partner with CamelBio for flexible, scalable sourcing of rare-target antibodies and customized bulk reagent solutions to accelerate your assay development pipeline.