Anti-TAX1BP3 Polyclonal Antibody for WB, ELISA - O14907

Core Product Specifications and Parameters

Biological Background: TAX1BP3 Function and Localization

• Tax1-binding protein 3 (TAX1BP3, also known as TIP-1 or glutaminase-interacting protein 3) is a scaffold protein that regulates protein-protein interactions by competing for PDZ domain binding sites.
• It binds to β-catenin (CTNNB1) and may act as an inhibitor of the Wnt signaling pathway.
• TAX1BP3 competes with LIN7A for binding to the potassium channel KCNJ4, promoting KCNJ4 internalization.
• A role in the Rho signaling pathway is suggested, potentially influencing cytoskeletal dynamics.
• The viral oncoprotein HPV16 E6 may utilize TAX1BP3 to activate the small GTPase CDC42.
• Subcellular localization includes the cytoplasm, nucleus, and cell membrane.
• The protein is ubiquitously expressed, with detection in brain, heart, kidney, lung, small intestine, skeletal muscle, and HeLa cells; weak expression in peripheral blood leukocytes.
• Post-translational modifications include acetylation and phosphorylation, as identified by proteomics.

Experimental Guidance and Technical Tips

• For Western blotting, expect a band near 14kDa. Use a low molecular weight marker for accurate sizing.
• HeLa cell lysates may serve as a suitable positive control given reported expression in this line.
• Validate antibody specificity in your target species (human/mouse) using knockdown or knockout samples if available.
• For ELISA, consider using the recombinant immunogen (aa 1-124) as a standard or coating antigen.
• If performing immunoprecipitation, note that TAX1BP3 is a small protein; confirm detection with a suitable loading control.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with one-stop access to IVD raw materials, including validated antibody pairs and optimized polyclonal antibodies such as this anti-TAX1BP3 antibody. Explore our portfolio for Wnt signaling and PDZ domain interaction targets, as well as bulk ancillary reagents. For rare target sourcing and tailored raw-material solutions, CamelBio bridges your concept-to-clinic needs efficiently.