Anti-Tau Rabbit Polyclonal Antibody for WB, IF/ICC, IF-P, IHC-P, ELISA - P10636

Core Product Specifications and Parameters

Biological Background: Tau Function and Localization

• Tau (microtubule-associated protein tau) promotes microtubule assembly and stability and is involved in the establishment and maintenance of neuronal polarity. Related references: PMID:21985311
• The protein acts as a linker between axonal microtubules (via its C-terminus) and neural plasma membrane components (via its N-terminus). Related references: PMID:21985311, PMID:32961270
• Short tau isoforms allow cytoskeletal plasticity, whereas longer isoforms preferentially stabilize the cytoskeleton.
• Tau localizes to the cytoplasm, cytosol, cytoskeleton, and cell membrane; it is present in axons, dendrites, and can be secreted.
• Expressed in neurons: the PNS-tau isoform is found in the peripheral nervous system, while other isoforms are expressed in the central nervous system.
• Tau undergoes extensive post-translational modifications (phosphorylation, acetylation, methylation, glycation, ubiquitination) and is a key player in Alzheimer disease and other tauopathies.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (aa 500-758) of human Tau, which includes the microtubule-binding repeat domains; consider this when interpreting isoform recognition.
• For Western blotting, the expected full-length band is ~79 kDa, but multiple isoforms between 45–68 kDa may also be detected depending on the sample.
• Brain tissue lysates are recommended for Western blot verification, as Tau is highly enriched in neurons.
• For immunohistochemical applications, validate antibody performance on relevant tissue sections and optimize antigen retrieval conditions as necessary.
• ELISA can be used to quantify Tau in biological fluids; however, appropriate controls and standard curves should be established.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers IVD manufacturers, diagnostic laboratories, and research institutes streamlined access to high-quality raw materials, including validated antibody pairs, recombinant proteins, and ancillary reagents. This Tau antibody supports neurodegenerative disease research and assay development, and CamelBio can assist in sourcing additional Tau-targeting antibodies, matched detection reagents, and bulk quantities to accelerate your project from concept to clinic.