Anti-Tau Monoclonal Antibody for WB - P10636

Core Product Specifications and Parameters

Biological Background: Tau Function and Localization

• Tau is a microtubule-associated protein that promotes assembly and stabilization of microtubules, critical for neuronal polarity. Related references: PMID:21985311
• It acts as a linker between axonal microtubules and neural plasma membrane components. Related references: PMID:21985311 PMID:32961270
• Alternative splicing generates multiple isoforms; shorter isoforms provide cytoskeletal plasticity, while longer isoforms favor stabilization.
• Tau is predominantly expressed in neurons, with CNS-specific isoforms and a peripheral nervous system isoform (PNS-tau).
• Subcellularly, tau localizes to the cytoplasm, cytoskeleton, axonal and dendritic projections, and is also associated with the cell membrane; it can be secreted.
• Post-translational modifications including phosphorylation, acetylation, methylation, glycation, and ubiquitination regulate tau function and are linked to pathological aggregation.
• Tau is the major component of paired helical filaments (PHF) and neurofibrillary tangles in Alzheimer's disease, and mutations in MAPT cause frontotemporal dementia and parkinsonism (FTDP-17).

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1-100) of human tau, which is present in all major isoforms. This antibody is suitable for detecting total tau in samples from human, mouse, and rat.
• For Western blot, the expected molecular weight is approximately 79 kDa, but multiple isoforms may appear as a band pattern between 48-68 kDa due to alternative splicing.
• In immunohistochemistry, ensure proper fixation and antigen retrieval, as tau is a cytosolic and cytoskeletal protein.
• For immunoprecipitation, validate the antibody's performance under native and denaturing conditions.
• Consider using phosphatase inhibitors during sample preparation to preserve phosphorylation states.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with one-stop access to high-quality IVD raw materials, including validated monoclonal antibodies like this anti-Tau antibody for neurodegenerative disease research. Our portfolio supports assay development with optimized antibody pairs, bulk ancillary reagents, and sourcing of rare targets. From concept to clinic, we streamline your supply chain for reliable diagnostic solutions.