Anti-TASL Rabbit Monoclonal Antibody for WB, IF/ICC, ELISA - Q9HAI6

Core Product Specifications and Parameters

Biological Background: TASL (CXorf21) Function and Localization

• TASL (also known as CXorf21) is an innate immune adapter that mediates IRF5 activation downstream of endolysosomal toll-like receptors TLR7, TLR8, and TLR9. Related references: PMID:32433612
• TASL is recruited to endolysosomes by SLC15A4 and specifically engages IRF5 via its pLxIS motif, triggering type I interferon production. Related references: PMID:32433612
• It also helps regulate endolysosomal pH in B-cells, dendritic cells, and monocytes. Related references: PMID:31001245
• TASL localizes to the lysosomal membrane, endosomal membrane, nucleus, and cytoplasm.
• Highly expressed in B-cells, neutrophils, dendritic cells, and monocytes, with 2–3-fold higher protein levels in female versus male cells; low expression in T-cells and NK cells.
• Classified as a phosphoprotein involved in innate immunity, TASL is a member of the reference proteome with available 3D structural data.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning amino acids 211–301; ensure your detection system is compatible with peptide-immunized rabbit monoclonal antibodies.
• For Western blot, the predicted molecular weight is ~34 kDa; verify band specificity using appropriate positive and negative controls in your sample system.
• In immunofluorescence/ICC, TASL exhibits lysosomal, endosomal, cytoplasmic, and nuclear localization; permeabilization and organelle-specific counterstains may help confirm subcellular distribution.
• For ELISA, consider pairing with a validated detection antibody and optimizing coating conditions using recombinant TASL or cell lysates.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines procurement for diagnostic manufacturers and research laboratories by offering a curated portfolio of IVD raw materials, including validated antibody pairs, optimized monoclonal antibodies like this anti-TASL rabbit mAb, and bulk ancillary reagents. Focusing on innate immunity and TLR signaling targets, we also source rare and emerging biomarker raw materials to accelerate your assay development from concept to clinic.