Anti-TAS2R38 Rabbit Polyclonal Antibody for WB, ELISA - P59533

Core Product Specifications and Parameters

Biological Background: TAS2R38 Function and Localization

• TAS2R38 belongs to the bitter taste receptor family T2R, serving as a G protein-coupled receptor (GPCR) that mediates the perception of bitterness, particularly for compounds like phenylthiocarbamide (PTC) (By similarity).
• Upon activation, TAS2R38 couples to gustducin, leading to downstream signaling via PLC-beta-2 and TRPM5 ion channel gating, ultimately transducing bitter taste stimuli (By similarity).
• Beyond the oral cavity, TAS2R38 may also function in the gastrointestinal tract, potentially sensing chemical composition of gut contents to modulate digestive responses (By similarity).
• The receptor is an integral membrane protein localized to the plasma membrane, featuring seven transmembrane helices characteristic of the GPCR superfamily.
• TAS2R38 is selectively expressed in gustducin-positive taste receptor cells on the tongue, and additionally found in testis tissue (PubMed:16720576). Related references: PMID:16720576
• As a glycoprotein, TAS2R38 undergoes post-translational glycosylation, which may influence its membrane trafficking and ligand binding properties.
• Genetic polymorphisms in the TAS2R38 gene give rise to different tasting phenotypes (e.g., taster vs. non-taster), impacting dietary preferences and potential health outcomes.

Experimental Guidance and Technical Tips

• The immunogen peptide corresponds to an internal sequence (aa 150–250) of human TAS2R38; this region may include intracellular or extracellular loops, so consider performing epitope accessibility tests in your assay format.
• When performing Western blotting, a band around 38 kDa is anticipated. Validate antibody specificity using appropriate positive and negative controls (e.g., tissue or cell lines known to express TAS2R38).
• Given the cross-reactivity with rat, this antibody may be suitable for detecting TAS2R38 in rat samples, but confirm cross-reactivity with your specific experimental model.
• For ELISA applications, optimize coating antigen and antibody dilution to achieve the desired sensitivity and specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For GPCR-focused studies, such as those investigating TAS2R38 in taste perception and sensory biology, CamelBio can supply validated antibody pairs, optimized recombinant proteins, and ancillary reagents to streamline your assay development. Let us be your strategic partner in sourcing high-quality raw materials for reliable and reproducible results.