Anti-TAS2R10 Polyclonal Antibody for WB, ELISA - Q9NYW0

Core Product Specifications and Parameters

Biological Background: TAS2R10 Function and Localization

• TAS2R10 (Taste receptor type 2 member 10), also known as taste receptor family B member 2 (TRB2) or T2R10, is a G protein-coupled receptor involved in bitter taste perception.
• It functions as a gustducin-coupled strychnine receptor, signaling through PLCB2 and the calcium-regulated cation channel TRPM5 upon ligand binding.
• TAS2R10 is expressed in subsets of taste receptor cells located in the tongue and palate epithelium, and exclusively in gustducin-positive cells.
• The receptor is localized to the plasma membrane and possesses seven transmembrane domains, characteristic of GPCRs.
• Post-translational glycosylation is predicted, consistent with its annotation as a glycoprotein.
• TAS2R10 is implicated in sensory transduction of bitter compounds not only in the oral cavity but also in the gastrointestinal tract.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a sequence within amino acids 100–200 of human TAS2R10, predicted to be in an extracellular or intracellular loop region, making it suitable for detecting the target in WB and ELISA.
• For Western blotting, the predicted molecular weight is approximately 35 kDa; however, glycosylation may result in a higher apparent molecular weight.
• For ELISA, consider using this antibody for antigen capture or detection in indirect ELISA formats; optimize coating concentrations and detection conditions.
• Cross-reactivity with mouse and rat indicates epitope conservation; validate performance in the relevant species sample system as needed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a one-stop IVD raw-material sourcing partner, providing validated antibody pairs, optimized polyclonal antibodies, and ancillary reagents to support taste receptor and sensory transduction research. For projects targeting TAS2R10 or other bitter taste receptors, we offer bulk antibody supply and custom sourcing solutions to streamline your IVD assay development with reliable, high-quality materials.