Anti-TAS1R2 Rabbit Polyclonal Antibody for WB and ELISA - Q8TE23

Core Product Specifications and Parameters

Biological Background: TAS1R2 Function and Localization

• TAS1R2 (Taste receptor type 1 member 2) is also referred to as G protein-coupled receptor 71 (GPR71) or sweet taste receptor T1R2.
• It forms a heterodimer with TAS1R3 to create the sweet taste receptor, responsible for detecting sweet compounds.
• This receptor recognizes diverse natural and synthetic sweeteners, playing a central role in sweet taste perception and sensory transduction.
• TAS1R2 is a G protein-coupled receptor featuring seven transmembrane domains and is classified under sensory transduction and signal transduction pathways.
• The protein localizes to the cell membrane, consistent with its function as a membrane-bound receptor.
• Post-translational modifications include glycosylation, as indicated by the Glycoprotein keyword.
• High-resolution structures (3D-structure keyword) are available, offering insights into its ligand-binding mechanisms.

Experimental Guidance and Technical Tips

• The immunogen used is a synthetic peptide positioned within the intracellular C-terminal region (amino acids 450–550). This suggests the antibody may detect the receptor in intracellular compartments or after membrane permeabilization.
• For Western blotting, consider using membrane protein extraction methods and optimizing sample preparation to account for the transmembrane nature of TAS1R2.
• Validate antibody performance in human tissue or cell lines known to express TAS1R2, such as taste bud cells or heterologous expression systems.
• In ELISA applications, peptide competition assays can help confirm binding specificity and assess antibody titer.
• Due to the high molecular weight (~95 kDa), allow sufficient migration time during SDS-PAGE for clear band separation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, supporting diagnostic manufacturers and research institutes with high-quality reagents from discovery to commercialization. This anti-TAS1R2 polyclonal antibody exemplifies our portfolio in taste receptor and sensory transduction research. Our supply capabilities include validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and assistance with rare target procurement to streamline your development workflows.