Anti-TARSL2 Rabbit Polyclonal Antibody for WB and ELISA - A2RTX5

Core Product Specifications and Parameters

Biological Background: TARSL2 Function and Localization

• TARSL2, also known as threonyl-tRNA synthetase (ThrRS) or threonyl-tRNA synthetase protein 3, is a cytoplasmic enzyme encoded by the TARS3 gene.
• It catalyzes the attachment of threonine to its cognate tRNA in a two-step reaction: activation of threonine by ATP to form Thr-AMP, followed by transfer to the acceptor end of tRNA(Thr).
• TARSL2 also possesses an editing domain to deacylate mischarged tRNA(Thr) at the post-transfer stage, ensuring translational fidelity.
• Subcellularly, TARSL2 localizes to both the cytoplasm and nucleus, consistent with its role in protein biosynthesis and potential nuclear functions.
• Key post-translational modifications include acetylation and phosphorylation, and the protein undergoes alternative splicing.
• Proteomics identification and direct protein sequencing have validated its expression and sequence, confirming its presence in the reference proteome.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1-170) of human TARSL2; consider this epitope location when designing detection assays or pairing with capture antibodies.
• This polyclonal antibody is suitable for Western blot (WB) and ELISA. For WB, a band around 93 kDa is expected. Validate antibody dilution and incubation conditions in your specific sample matrix.
• Cross-reactivity with mouse and rat TARSL2 has been indicated; verify performance in your target species and consider sequence alignment to confirm epitope conservation.
• For ELISA, pairing with a matched detection antibody may improve sensitivity; perform checkerboard titrations to determine optimal concentrations.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with streamlined access to high-quality IVD raw materials, including validated antibody pairs, monoclonal and polyclonal antibodies, and bulk ancillary reagents. This anti-TARSL2 polyclonal antibody, developed against the N-terminus of the human threonine--tRNA ligase, supports applications in protein biosynthesis and aminoacyl-tRNA synthetase research. Whether you need existing catalog products or custom rare-target sourcing, CamelBio bridges the gap from discovery to diagnostic development with reliable supply and technical support.