Anti-TARS2 Polyclonal Antibody for WB, ELISA - Q9BW92

Core Product Specifications and Parameters

Biological Background: TARS2 / Threonine--tRNA ligase, mitochondrial Function and Localization

• TARS2 encodes mitochondrial threonine-tRNA ligase, also known as threonyl-tRNA synthetase (ThrRS) or TARSL1, essential for mitochondrial protein synthesis.
• Catalyzes the ATP-dependent attachment of threonine to its cognate tRNA(Thr) and performs proofreading through its editing domain to remove mischarged amino acids.
• Contains a transit peptide directing its import into mitochondria; the mature enzyme localizes to the mitochondrial matrix.
• Belongs to the class II aminoacyl-tRNA synthetase family; mutations are associated with combined oxidative phosphorylation deficiency 21 (COXPD21), a mitochondrial disease.
• Undergoes alternative splicing, producing multiple transcript variants, and is phosphorylated at several sites.
• Key biological keywords: aminoacyl-tRNA synthetase, ligase, mitochondrial protein biosynthesis, nucleotide-binding, phosphoprotein.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 100–400 of human TARS2, a region spanning part of the catalytic core; the antibody likely recognizes epitopes within this sequence.
• For Western blot, mitochondrial-enriched fractions or whole-cell lysates from human, mouse, and rat cell lines can be probed at the expected molecular weight of 81 kDa; consider using a mitochondrial marker for co-detection.
• In ELISA, the antibody may be used as capture or detection reagent; pairing with another TARS2 antibody might improve sandwich ELISA performance if validated.
• Because TARS2 is a low-abundance mitochondrial enzyme, signal amplification steps or increased exposure times might be beneficial.
• Validate specificity in target-knockdown or knockout cell models, or via peptide competition assays, to confirm the band identity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated provider of IVD raw materials, supporting diagnostic manufacturers and research laboratories in mitochondrial biology fields with high-quality antibodies. This TARS2 antibody complements our portfolio of validated reagents for translational and metabolic disease research. We offer one-stop access to customized bulk antibodies, ancillary reagents, and rare target sourcing to streamline your assay development from concept to clinic.