Anti-TARS Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P26639

Core Product Specifications and Parameters

Biological Background: Threonine--tRNA Ligase 1 Function and Localization

• Threonine--tRNA ligase 1, cytoplasmic (TARS, also known as TARS1, ThrRS), is a member of the aminoacyl-tRNA synthetase family that specifically charges tRNA(Thr) with threonine.
• Catalyzes the attachment of threonine to tRNA(Thr) via a two-step mechanism: threonine activation by ATP to form Thr-AMP, followed by transfer to the acceptor end of tRNA(Thr) (PubMed:25824639, PubMed:31374204). Related references: PMID:25824639, PMID:31374204
• Possesses an editing domain that hydrolytically removes mischarged amino acids from tRNA(Thr) at the post-transfer stage, ensuring high fidelity of protein synthesis (By similarity).
• Localizes predominantly to the cytoplasm, where it functions in translational elongation.
• Subject to multiple post-translational modifications including acetylation, phosphorylation, and ubiquitin-like conjugation; also exhibits alternative splicing.
• Contains disease-associated variants (keyword: Disease variant), implicating TARS in human pathology.
• Structural studies (3D-structure keyword) have provided insights into its catalytic and editing domains.

Experimental Guidance and Technical Tips

• The antibody is raised against a recombinant immunogen corresponding to the N-terminal region (amino acids 1–230) of human TARS. Confirm reactivity with your target of interest in relevant species (human, mouse, rat).
• For Western blot (WB), use appropriate protein loading controls and validate band specificity using knockdown or knockout cell lysates if available.
• In immunohistochemistry (IHC-P), optimize antigen retrieval and antibody dilution; include positive and negative tissue controls.
• For immunofluorescence (IF/ICC), consider fixation and permeabilization conditions suitable for cytoplasmic targets.
• The polyclonal nature may recognize multiple isoforms or modified forms; validate in your experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive one-stop partner for IVD raw-material sourcing, technical support, and consultative services from concept to clinic. This anti-TARS polyclonal antibody is ideal for research in protein translation, aminoacyl-tRNA synthetase biology, and related disease areas. Beyond individual antibodies, CamelBio offers validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets to support your diagnostic and research pipelines.