Anti-TARBP1 Rabbit Polyclonal Antibody for WB, ELISA - Q13395

Core Product Specifications and Parameters

Biological Background: TARBP1 Function and Localization

• TARBP1 is a tRNA (guanosine(18)-2'-O)-methyltransferase that selectively catalyzes 2'-O-methylguanosine at position 18 (Gm18) in tRNAGln-TTG/CTG and tRNASer-TGA/GCT, enhancing tRNA stability. Related references: PMID:18412263 PMID:31019095 PMID:38867004
• In the context of HIV-1 infection, TARBP1 binds the TAR RNA loop structure and competes with RNA polymerase II, potentially disengaging the polymerase from viral RNA to regulate transcription. Related references: PMID:7638159 PMID:8626763 PMID:8846792
• Post-translational modifications of TARBP1 include acetylation and phosphorylation, which may regulate its activity or interactions.
• TARBP1 contains S-adenosyl-L-methionine-dependent methyltransferase and RNA-binding domains, classifying it as a member of the methyltransferase superfamily involved in tRNA processing.

Experimental Guidance and Technical Tips

• Because the immunogen targets the C-terminal region (aa 1322-1621) of human TARBP1, consider using this fragment as a positive control in competitive assays or epitope mapping studies.
• For Western blot applications, given the high predicted molecular weight of approximately 182 kDa, ensure prolonged transfer and use of high-molecular-weight markers. Validate the antibody with appropriate lysates and controls.
• In ELISA, the antibody can be employed either as a capture or detection reagent for native or recombinant TARBP1; optimal working dilutions should be determined empirically.
• Cross-reactivity is reported for human; when applying this antibody to other species, perform sequence alignment and experimental validation to confirm specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, offering diagnostic manufacturers and research groups access to validated antibodies, bulk ancillary reagents, and rare-target material procurement. For RNA biology and virology research fields—such as studies on tRNA modification pathways or HIV-1 transcriptional regulation—CamelBio streamlines supply chains with one-stop sourcing for polyclonal antibodies like this anti-TARBP1 rabbit pAb, as well as monoclonal antibodies and recombinant proteins tailored to your assay development needs.