Anti-TAP1 Monoclonal Antibody for WB, ELISA - Q03518

Core Product Specifications and Parameters

Biological Background: TAP1 Function and Localization

• TAP1 (Antigen peptide transporter 1), also known as ABCB2, PSF1, and RING4, forms a heterodimer with TAP2 to function as an ATP-dependent peptide transporter that translocates cytosolic peptides into the endoplasmic reticulum for MHC class I loading (PubMed:25377891, PubMed:25656091). Related references: PMID:25377891 PMID:25656091

• The transporter preferentially selects peptides of 8–13 amino acids with free N- and C-termini; a hydrophobic residue at position 3 and a hydrophobic or charged C-terminal anchor are favored, while proline at position 2 is destabilizing (PubMed:11274390, PubMed:7500034, PubMed:9256420). Related references: PMID:11274390 PMID:7500034 PMID:9256420

• TAP1 is a critical component of the peptide loading complex (PLC), acting as a molecular scaffold that facilitates peptide-MHCI assembly and antigen presentation (PubMed:1538751, PubMed:25377891, PubMed:26611325). Related references: PMID:1538751 PMID:25377891 PMID:26611325

• Subcellular localization is restricted to the endoplasmic reticulum membrane, consistent with its role in peptide translocation.

• Highly expressed in professional antigen-presenting cells (monocytes, dendritic cells) and lymphocyte subsets (T cells, B cells, NK cells), underscoring its importance in adaptive immunity.

Experimental Guidance and Technical Tips

• The immunogen encompasses the C-terminal region (a.a. 449–748) containing part of the nucleotide-binding domain, which is conserved and supports reactivity with both human and mouse TAP1 in WB and ELISA.
• For Western blotting, validate the antibody with TAP1-expressing cell lines (e.g., Jurkat, Raji) and optimize sample preparation; the predicted molecular weight is 81 kDa, though post-translational modifications may shift migration.
• In ELISA, consider using the antibody as a capture or detection reagent; pairing with a TAP2 antibody or using recombinant TAP1 standards may improve quantification.
• TAP1 expression is inducible by immune stimulation; account for potential upregulation in activated cells during experimental design.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your single-source partner for IVD raw materials, providing monoclonal antibodies like this TAP1 antibody to advance immunology and antigen-processing research. We supply validated antibody pairs, bulk reagents, and rare-target sourcing, supporting your development from concept to clinic.