Anti-TAOK2 Polyclonal Antibody for WB and ELISA - Q9UL54

Core Product Specifications and Parameters

Biological Background: TAOK2 Function and Localization

• Encodes serine/threonine-protein kinase TAO2, also known as prostate-derived STE20-like kinase 1 (PSK1) and thousand and one amino acid protein kinase 2. Isoforms 1 and 2 are produced by alternative splicing.
• Functions in stress-activated MAPK cascades: phosphorylates MAP2K3 and MAP2K6 to activate p38/MAPK14, and participates in DNA damage checkpoint control at the G2/M transition.
• Isoform 1 induces apoptotic morphological changes (cell contraction, membrane blebbing, apoptotic body formation) via MAPK8/JNK and mitochondrial CASP9 pathways; localizes to the nucleus when C-terminally truncated.
• Isoform 1 binds microtubules and modulates their organization and stability independently of kinase activity. Prevents MAP3K7-mediated NF-kappa-B activation but not MAPK8/JNK activation.
• Isoform 2 is required for PCDH8 endocytosis. Upon homophilic PCDH8 interactions, isoform 2 phosphorylates p38/MAPK14, leading to reciprocal phosphorylation and internalization of PCDH8 and CDH2.
• Subcellular locations include cytoplasmic vesicle membrane, cytoplasm, cytoskeleton, nucleus, and cell projection dendrites. Ubiquitously expressed, with higher levels in testis and brain.
• Belongs to the protein kinase superfamily and contains signature motifs for ATP binding, magnesium coordination, and phosphotransferase activity.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a recombinant fragment spanning amino acids 610–730 of human TAOK2 (NP_057235.2), a region within the kinase domain that may confer isoform selectivity. Verify recognition of both full-length and processed isoforms in your model.
• For Western blot, the predicted molecular weight is approximately 138 kDa. Use appropriate gel percentages and transfer conditions for high-molecular-weight proteins.
• In ELISA, consider pairing with a detection antibody against a different epitope to avoid competition. Validate specificity using knockout or knockdown controls.
• Because TAOK2 functions in stress and DNA damage pathways, induce relevant stimuli (e.g., osmotic stress, genotoxic agents) when assessing activation-dependent phosphorylation or localization changes.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines procurement for diagnostic manufacturers, research labs, and institutes by offering validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing. For studies on cell stress, apoptosis, or neuronal signaling, our TAOK2-related products—including this rabbit polyclonal antibody—can support your assay development and biomarker discovery programs. Contact us to discuss custom sourcing solutions tailored to your pipeline.