Anti-TAOK1 Rabbit Monoclonal Antibody for WB, ELISA - Q7L7X3

Core Product Specifications and Parameters

Biological Background: TAOK1 Function and Localization

• TAOK1 (Thousand and one amino acid protein kinase 1), also known as PSK2, MARKK, or MAP3K16, is a serine/threonine-protein kinase.
• It acts as a key activator of the p38/MAPK14 stress-activated MAPK cascade by phosphorylating upstream MAP2K3 and MAP2K6 kinases, participating in cellular stress responses and DNA damage-induced G2/M checkpoint control.
• Through phosphorylation of MARK2 at Thr-208, TAOK1 regulates cytoskeleton stability and microtubule dynamics, influencing processes like cell polarity and migration.
• It contributes to apoptotic programmed cell death by activating the MAPK8/JNK pathway, leading to cell contraction, membrane blebbing, and apoptotic body formation.
• TAOK1 plays an essential role in neuronal development and cortical neuronal migration (PubMed:33565190). Related references: PMID:33565190
• The protein localizes predominantly to the cytoplasm and is highly expressed in testis, with lower expression in brain, placenta, colon, and skeletal muscle.
• TAOK1 is a phosphoprotein undergoing alternative splicing, associated with disease variants linked to intellectual disability, and features a kinase domain and coiled-coil regions.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 900–985 near the C-terminus; the antibody may recognize full‑length TAOK1 and isoforms containing this region.
• For western blotting, the predicted molecular weight is ~116 kDa; consider using a 6–8% SDS-PAGE gel and extended transfer times for high‑molecular‑weight proteins.
• Validate reactivity in the relevant sample system (human, mouse, rat) using appropriate positive and negative controls.
• For ELISA, optimize coating antigen concentration and detection conditions empirically; include a standard curve for quantitative assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutions with comprehensive access to IVD raw materials, supporting projects from concept to clinic. For targets like TAOK1 critical in cell signaling, neurodevelopment, and oncology, we offer validated antibody pairs, optimized monoclonal antibodies, and bulk ancillary reagents, as well as assistance in sourcing rare reagents to accelerate your assay development.