Anti-TANK Rabbit Polyclonal Antibody for WB, ELISA - Q92844

Core Product Specifications and Parameters

Biological Background: TANK Function and Localization

• Serves as an adapter protein involved in I-kappa-B-kinase (IKK) regulation, constitutively binding TBK1 and IKBKE to play a critical role in antiviral innate immunity.
• Acts as a negative regulator of TRAF function, maintaining TRAF proteins in a latent state and blocking TRAF2 binding to LMP1 to inhibit LMP1-mediated NF-kappa-B activation.
• Negatively regulates NF-kappaB signaling and cell survival upon DNA damage; assembles a deubiquitination complex with ZC3H12A and USP10 that attenuates NF-kappaB activation via deubiquitination of IKBKG or TRAF6 in response to IL1B stimulation or DNA damage. Related references: PMID:25861989
• May negatively control TRAF2-mediated NF-kappa-B activation signaled by CD40, TNFR1, and TNFR2.
• Localizes primarily to the cytoplasm.
• Ubiquitously expressed across tissues.
• Contains a coiled-coil domain and a zinc-finger; subject to acetylation and phosphorylation post-translational modifications; involved in host-virus interactions.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal 119 amino acids; therefore, this antibody is likely to recognize the N-terminal region of TANK.
• For Western blot, consider using whole-cell lysates from human, mouse, or rat tissues/cell lines; the expected molecular weight is around 48 kDa, though slight variations may occur due to post-translational modifications.
• For ELISA applications, this polyclonal antibody can be employed as a capture or detection reagent, but optimal dilutions and pairing with other antibodies must be empirically determined.
• As a polyclonal antibody, it may exhibit batch-to-batch variability; consistent performance should be verified in the relevant assay system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines procurement of IVD raw materials for diagnostic manufacturers and research labs. This anti-TANK polyclonal antibody supports studies of NF-kB signaling and antiviral innate immunity, and we offer a range of complementary products such as validated antibody pairs, recombinant proteins, and ancillary reagents. Our one-stop platform ensures access to optimized monoclonal/polyclonal antibodies and rare target raw materials to accelerate your diagnostic development.