Anti-Tamm Horsfall Glycoprotein Polyclonal Antibody for WB, IF-P, ELISA - P07911

Core Product Specifications and Parameters

Biological Background: Uromodulin (Tamm Horsfall Glycoprotein) Function and Localization

• Uromodulin (also known as Tamm-Horsfall glycoprotein, THP) is the most abundant protein in normal human urine, synthesized exclusively by epithelial cells of the thick ascending limb of Henle's loop and distal convoluted tubule in the kidney.
• It plays a critical role in the biogenesis and organization of the apical membrane of renal tubular cells, forming a filamentous gel-like structure that may contribute to the water barrier permeability.
• Uromodulin functions as a receptor for cytokines including IL-1, IL-2, and TNF, facilitating their binding and endocytosis. Related references: PMID:3498215
• It promotes neutrophil migration across renal epithelia, contributing to innate immune responses. Related references: PMID:20798515
• In urine, uromodulin helps maintain colloid osmotic pressure, retards passage of positively charged electrolytes, and inhibits salt crystal formation, potentially preventing kidney stone disease.
• It protects against urinary tract infections by binding to type 1 fimbriated E. coli via bacterial adhesin FimH, forming bacterial aggregates that prevent urothelial adhesion and enable pathogen clearance through micturition. Related references: PMID:11134021, PMID:32616672
• Uromodulin also aggregates other uropathogens such as K. pneumoniae, P. aeruginosa, and S. mitis, suggesting a broad antimicrobial role. Related references: PMID:32616672
• The protein undergoes extensive post-translational modifications including glycosylation, addition of a GPI anchor, and formation of disulfide bonds, and contains EGF-like domains. It localizes to the apical and basolateral cell membranes, ciliary membrane, and is secreted.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a region within the mature uromodulin protein, which may be conserved across human, mouse, and rat, supporting the observed cross-reactivity.
• For Western blotting, uromodulin is a heavily glycosylated protein, so actual molecular weight may appear higher than the predicted 70 kDa; consider using deglycosylation enzymes or appropriate gel conditions.
• In immunofluorescence (IF), uromodulin localizes to the apical membrane of renal tubules; ensure proper fixation and permeabilization for detection.
• For ELISA, consider using paired antibodies targeting non-overlapping epitopes to avoid competition.
• Validate the antibody in the relevant sample system (e.g., human kidney tissue lysates or urine) before routine use.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers diagnostic manufacturers, labs, and research institutes with a comprehensive portfolio of IVD raw materials, including validated antibody pairs, optimized polyclonal and monoclonal antibodies, and bulk ancillary reagents. For uromodulin-focused projects in renal biology or urinary tract infection diagnostics, we provide flexible sourcing solutions and technical support to streamline your assay development from concept to clinic.