Anti-Talin 2 Rabbit Monoclonal Antibody for WB, IF-P, IHC-P, ELISA - Q9Y4G6

Core Product Specifications and Parameters

Biological Background: Talin-2 Function and Localization

• Talin-2 (encoded by TLN2) is a large (~272 kDa) cytoskeletal scaffold protein and a core component of focal adhesion plaques.
• It functions as a critical linker between integrin adhesion receptors and the actin cytoskeleton, regulating cell adhesion, spreading, and migration.
• Talin-2 recruits phosphatidylinositol-4-phosphate 5-kinase type-1 gamma (PIP5K1C) to focal adhesions and potently activates its kinase activity, thereby modulating local PIP2 levels and downstream signaling.
• Subcellularly, Talin-2 localizes to the cytoplasm, cell membrane, and specialized adhesion structures such as focal adhesions and synapses; it also associates with the actin cytoskeleton.
• Post‑translational modifications include phosphorylation (phosphoprotein), and its three‑dimensional structure has been resolved (3D‑structure keyword).
• Talin-2 is part of the human reference proteome and has been identified in multiple proteomics studies, highlighting its ubiquitous and functional importance.

Experimental Guidance and Technical Tips

• The immunogen lies within amino acids 350–450 of human Talin-2; therefore, the antibody may detect N‑terminal truncated isoforms. Validate the target band(s) in your sample.
• For western blotting, use a low‑percentage SDS‑PAGE gel (e.g., 6–8%) and extended transfer times to ensure efficient detection of the high‑molecular‑weight (272 kDa) Talin‑2 protein.
• In immunofluorescence and immunohistochemistry, include a permeabilization step, as Talin‑2 is a cytosolic and cytoskeleton‑associated protein. Colocalization with focal adhesion markers (e.g., vinculin, paxillin) is recommended.
• The antibody cross‑reacts with human, mouse, and rat samples; however, performance should be confirmed in the specific experimental context (e.g., cell type, tissue fixation conditions).
• For ELISA applications, pair the antibody with a matched detection reagent (e.g., an anti‑Talin‑2 polyclonal antibody) after validating binding linearity and specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with a reliable, one‑stop channel for IVD raw materials, technical services, and consulting. For researchers investigating cell adhesion, focal adhesion dynamics, and cytoskeletal organization, we supply validated antibody pairs, optimized monoclonal/polyclonal antibodies (including rabbit monoclonal Talin‑2 antibodies), bulk ancillary reagents, and can assist in sourcing rare‑target raw materials. Whether you need a single reagent or an integrated supply solution from concept to clinic, contact us to streamline your procurement and development.