Anti-Talin-1 Polyclonal Antibody for WB, IF-P, ELISA - Q9Y490

Core Product Specifications and Parameters

Biological Background: Talin-1 Function and Localization

• Talin-1 is a high molecular weight cytoskeletal protein encoded by the TLN1 gene (also known as KIAA1027, TLN) in humans.
• It is concentrated at regions of cell-substratum and cell-cell contacts, serving as a critical linker between the actin cytoskeleton and integrin receptors at focal adhesions.
• Talin-1 plays a key role in mechanically coupling the cytoskeleton to the extracellular matrix, facilitating signal transduction and cell adhesion.
• In collaboration with KANK1, Talin-1 co-organizes cortical microtubule stabilizing complexes (CMSCs) at focal adhesion rims to regulate microtubule-actin crosstalk.
• Subcellularly, Talin-1 localizes to the cytoplasm, cytoskeleton, cell surface, cell junctions, focal adhesions, and cell projections such as ruffle membranes.
• Talin-1 is expressed in many tissues, with particularly high expression in skin and pancreas, and robust expression in kidney cortex, endocervix, testis, pituitary, liver, and spleen.
• The protein undergoes multiple post-translational modifications including acetylation and phosphorylation, and is involved in host-virus interactions.

Experimental Guidance and Technical Tips

• For Western blot analysis, consider using a low percentage gel (e.g., 6–8%) and extended transfer time to accommodate the high molecular weight (~270 kDa) of Talin-1.
• In immunofluorescence/IF-P, ensure adequate permeabilization of cells to allow antibody access to focal adhesion sites. Co-staining with focal adhesion markers is recommended to confirm localization.
• For ELISA applications, use the recombinant immunogen (N-terminal 1-220) as a positive control and validate the antibody with your specific sample matrix.
• Cross-reactivity with human, mouse, and rat has been reported; confirm performance in your species and tissue of interest.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with one-stop access to validated Talin-1 antibodies, antigen proteins, and ancillary raw materials essential for cell adhesion and oncology biomarker studies. Our sourcing network can supply bulk polyclonal antibodies, matched antibody pairs, and rare-target reagents to streamline your assay development from concept to clinic. Partner with CamelBio for reliable, scalable raw-material solutions in IVD development.