Anti-TALDO1 Polyclonal Antibody for WB, ELISA - P37837

Core Product Specifications and Parameters

Biological Background: TALDO1 Function and Localization

• Transaldolase (TALDO1) catalyzes the rate-limiting step of the non-oxidative phase in the pentose phosphate pathway, reversibly converting sedoheptulose-7-phosphate and D-glyceraldehyde 3-phosphate into erythrose-4-phosphate and fructose-6-phosphate. Related references: PMID:18687684 PMID:8955144
• Beyond its canonical enzymatic role, TALDO1 influences additional metabolic networks through dynamic shuttling between the nucleus and the cytoplasm.
• Subcellular localization includes both the nucleus and cytoplasm, with translocation events linked to non-metabolic functions such as transcriptional regulation.
• The protein undergoes post-translational modifications including acetylation and phosphorylation, which may modulate its activity or localization.
• Encoded by the TALDO1 gene (aliases TAL, TALDO, TALDOR), the primary human protein comprises 337 amino acids with a calculated molecular weight of approximately 37.5 kDa (observed at ~38 kDa).
• As a class I aldolase, it forms a Schiff base intermediate during catalysis, a hallmark of the transaldolase family.
• Alternative translation initiation generates multiple isoforms, potentially contributing to functional diversity.
• UniProt links TALDO1 deficiency to a rare inborn error of metabolism, underlining its clinical significance.

Experimental Guidance and Technical Tips

• The immunogen covers the full-length human TALDO1 protein (aa 1–337), suggesting the antibody may detect all isoforms; confirm isoform specificity in your model system.
• For Western blotting, use standard SDS-PAGE conditions and verify band identity with positive (e.g., HeLa lysate) and negative controls. Start with a broad dilution series to establish optimal working concentration.
• ELISA performance may vary with coating conditions and detection substrates; consider checkerboard titrations to optimize signal-to-noise ratio.
• The listed cross-reactivity (human, mouse, rat) relies on sequence homology and supplier testing—validate in your specific sample types and applications.
• To interrogate TALDO1’s nuclear–cytoplasmic shuttling, consider subcellular fractionation or immunofluorescence alongside total lysate analysis.
• Pre-absorption with the immunogen can serve as a specificity control; this is especially important when probing tissues with high endogenous transaldolase activity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers diagnostic manufacturers and research laboratories with a comprehensive portfolio of IVD raw materials, including validated antibody pairs, monoclonal and polyclonal antibodies, and bulk ancillary reagents. This high-quality anti-TALDO1 polyclonal antibody supports critical investigations into the pentose phosphate pathway, metabolic disorders, and related disease mechanisms. For rare target sourcing or custom development needs, our integrated technical and supply chain services provide a reliable bridge from concept to clinical application.