Anti-TAL1 Polyclonal Antibody for WB, ELISA - P17542

Core Product Specifications and Parameters

Biological Background: TAL1 Function and Localization

• T-cell acute lymphocytic leukemia protein 1 (TAL1), also known as SCL, TCL5, and bHLHa17, is a class A basic helix-loop-helix (bHLH) transcription factor encoded by the TAL1 gene.
• TAL1 is implicated in the genesis of hemopoietic malignancies and serves as a positive regulator of erythroid differentiation (By similarity).
• It belongs to the bHLH family of transcription factors, which play critical roles in developmental processes and cell lineage specification.
• TAL1 localizes to the nucleus, consistent with its function as a DNA-binding transcriptional regulator.
• The protein undergoes alternative splicing and post-translational modifications, including phosphorylation and ubiquitin conjugation, which may modulate its activity.
• TAL1 is specifically expressed in leukemic stem cells, underscoring its involvement in hematological cancers.
• Chromosomal rearrangements involving the TAL1 gene are a hallmark of T-cell acute lymphoblastic leukemia (T-ALL), classifying it as a proto-oncogene.
• Structural studies (3D-structure keyword) and proteomic identification efforts have provided insights into its molecular interactions.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 242-331 of human TAL1 (NP_003180.1), covering the C-terminal region containing the bHLH domain; consider epitope mapping if cross-species reactivity is observed.
• For Western blotting, the predicted molecular weight of TAL1 is 34 kDa. Use appropriate positive and negative controls from human or rat cell lines expressing TAL1.
• In ELISA, the antibody can be used for detecting TAL1 in recombinant protein or cell lysates; optimize coating and detection conditions for your assay.
• As TAL1 is a nuclear protein, employ efficient nuclear extraction buffers to enhance detection sensitivity in immunoblotting.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers comprehensive IVD raw material procurement, supporting diagnostics manufacturers from concept to clinic. The anti-TAL1 polyclonal antibody is part of our portfolio for hemopoiesis and leukemia research. We provide validated antibody pairs, bulk monoclonal and polyclonal antibodies, and ancillary reagents, along with rare target sourcing to accelerate your assay development.